Cross-talk between integrin receptor and insulin-like growth factor receptor in regulation of collagen biosynthesis in cultured fibroblasts

• Published in: Advances in medical sciences 2013-12, Vol.58 (2), p.292-297
• Main Authors: Prokop, I, Konończuk, J, Surażyński, A, Pałka, J
• Format: Article
• Language: English
• Subjects: Butadienes - pharmacology; Cell Line; Chromones - pharmacology; Collagen; Collagen - biosynthesis; Dipeptidases - metabolism; Enzyme Inhibitors - pharmacology; Fibroblasts - cytology; Fibroblasts - drug effects; Fibroblasts - metabolism; Flavonoids - pharmacology; Humans; IGF-IR; IGFBP-1; Insulin-Like Growth Factor Binding Protein 1 - pharmacology; Insulin-Like Growth Factor I - pharmacology; Integrin beta1 - pharmacology; Morpholines - pharmacology; Nitriles - pharmacology; Peptides - pharmacology; Phosphorylation - drug effects; Phosphorylation - physiology; Prolidase; Receptor Cross-Talk - drug effects; Receptor Cross-Talk - physiology; Receptor, IGF Type 1 - metabolism; Signal Transduction - drug effects; Signal Transduction - physiology; Skin - cytology; Thrombin - pharmacology; β1-integrins
• ISSN: 1896-1126; 1898-4002
• DOI: 10.2478/v10039-012-0072-0

Cellular processes are regulated by signals generated by adhesion receptors and growth factor receptors. IGF-binding protein 1 (IGFBP-1) is a molecule which may affect the both signaling pathways through inactivation of IGF-I (ligand for IGF-IR) and binding to RGD region of integrin receptors. Whether this phenomenon is important in communication between insulin-like growth factor receptor (IGF-IR) and β1-integrin receptor in regulation of prolidase activity and collagen biosynthesis is the aim of this study. We studied the effects of IGFBP-1, IGF-I, thrombin (integrin activator), echistatin (disintegrin), phosphatidylinositol 3-kinase inhibitor (LY-294002) and ERK 1/2 inhibitors (PD98059 and UO126) on prolidase activity, collagen biosynthesis and expression of proteins participating in pathways generated by these receptors. Stimulation of β1-integrin and IGF-I receptors by standard ligands was proved to up-regulate collagen synthesis in cultured fibroblasts. IGFBP-1, similarly as echistatin and studied inhibitors, contributed to down-regulation of ERK1/2, Akt, mTOR expression and up-regulation of NFκB. It was accompanied by parallel decrease in prolidase activity and collagen biosynthesis. The data suggest that “cross talk” between IGF-I receptor and integrin receptor may play important role in regulation of prolidase activity and collagen biosynthesis.