Prevention of H2O2-induced oxidative stress in Chang liver cells by 4-hydroxybenzyl-chitooligomers

• Published in: Carbohydrate polymers 2014-03, Vol.103, p.502-509
• Main Authors: Trinh, Mai Duy Luu, Ngo, Dai-Hung, Tran, Dang-Khoa, Tran, Quoc-Tuan, Vo, Thanh-Sang, Dinh, Minh-Hiep, Ngo, Dai-Nghiep
• Format: Article
• Language: English
• Subjects: 4-Hydroxybenzyl-chitooligomers (HB-COS); Antioxidant enzymes; Antioxidants - chemical synthesis; Antioxidants - chemistry; Antioxidants - pharmacology; Applied sciences; Benzhydryl Compounds - chemistry; Benzhydryl Compounds - pharmacology; Biological and medical sciences; Cell Line; Chang liver cells; Chitosan - chemistry; Chitosan - pharmacology; DNA - drug effects; DNA - metabolism; Exact sciences and technology; Free radical scavenging; General and cellular metabolism. Vitamins; Humans; Hydrogen Peroxide - antagonists & inhibitors; Hydrogen Peroxide - pharmacology; Medical sciences; Natural polymers; NF-kappa B - antagonists & inhibitors; NF-kappa B - metabolism; Nuclear factor kappa B (NF-κB); Oxidative Stress - drug effects; Pharmacology. Drug treatments; Physicochemistry of polymers; Reactive Oxygen Species - antagonists & inhibitors; Reactive Oxygen Species - metabolism; Signal Transduction - drug effects; Starch and polysaccharides
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2013.12.061

•Intracellular free radicals scavenging activities of 4-hydroxybenzyl-chitooligomers (HB-COS) were determined in Chang liver cells.•HB-COS exerted protective effects against reactive oxygen species and DNA oxidation.•HB-COS increased the gene and protein expressions of intracellular antioxidant enzymes.•Antioxidant effects are due to suppression of NF-κB signaling pathway.•Collectively, HB-COS can be used as a free radicals scavenger in cellular system. In this study, a bioactive derivative of chitooligomers (1.0–3.0kDa), 4-hydroxybenzyl-COS (HB-COS), was synthesized to enhance antioxidant activity. Hence, HB-COS was evaluated for its capabilities against H2O2-induced oxidative stress in human Chang liver cells. It was found that HB-COS possessed the free radical scavenging activity via decreasing the intracellular reactive oxygen species production. Furthermore, HB-COS significantly reduced the oxidation of DNA in a dose-dependent manner. Notably, HB-COS treatment upregulated the gene and protein expressions of antioxidative enzymes and thereby enhancing the intracellular antioxidant mechanisms. In addition, HB-COS treatment caused a remarkable blockade on degradation of inhibitory kappa B alpha (IκB-α) protein and translocation of nuclear factor kappa B (NF-κB). The current study demonstrated that HB-COS effectively attenuated hydrogen peroxide-induced oxidative stress in Chang liver cells by increasing levels of antioxidant enzymes and inhibiting reactive oxygen species generation, DNA oxidation and the NF-κB signaling pathway.