Porphyrin conjugated with serum albumins and monoclonal antibodies boosts efficiency in targeted destruction of human bladder cancer cells

The synthesis of a novel PS conjugated with bovine and human serum albumin (BSA and HSA) and a monoclonal antibody anti-CD104 is reported, as well as their biological potential against the human bladder cancer cell line UM-UC-3. No photodynamic effect was detected when the non-conjugated porphyrin w...

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Bibliographic Details
Published in:Organic & biomolecular chemistry 2014-03, Vol.12 (11), p.1804-1811
Main Authors: Pereira, Patrícia M R, Carvalho, José J, Silva, Sandrina, Cavaleiro, José A S, Schneider, Rudolf J, Fernandes, Rosa, Tomé, João P C
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal - chemistry
Cell Line, Tumor
Enzyme-Linked Immunosorbent Assay
Humans
Photochemotherapy
Photosensitizing Agents - chemistry
Photosensitizing Agents - therapeutic use
Porphyrins - chemistry
Reactive Oxygen Species - metabolism
Serum Albumin - chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Urinary Bladder Neoplasms - drug therapy
Urinary Bladder Neoplasms - metabolism
Urinary Bladder Neoplasms - pathology
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Summary:The synthesis of a novel PS conjugated with bovine and human serum albumin (BSA and HSA) and a monoclonal antibody anti-CD104 is reported, as well as their biological potential against the human bladder cancer cell line UM-UC-3. No photodynamic effect was detected when the non-conjugated porphyrin was used. Yet, when it was coupled covalently with the mAb anti-CD104, BSA and HSA, the resulting photosensitizer conjugates demonstrated high efficacy in destroying the cancer cells, the mAb anti-CD104 efficacy overruling the albumins.
ISSN:1477-0520
1477-0539
DOI:10.1039/c3ob42082e