Effect of E‑Beam Treatment on the Chemistry and on the Antioxidant Activity of Lycopene from Dry Tomato Peel and Tomato Powder

• Published in: Journal of agricultural and food chemistry 2014-02, Vol.62 (7), p.1557-1563
• Main Authors: Gámez, M. Carmen, Calvo, Marta M, Selgas, M. Dolores, García, M. Luisa, Erler, Katrin, Böhm, Volker, Catalano, Assunta, Simone, Rossella, Palozza, Paola
• Format: Article
• Language: English
• Subjects: Animals; antioxidant activity; Antioxidants - chemistry; Antioxidants - pharmacology; Carotenoids - chemistry; Carotenoids - pharmacology; cell culture; Cell Line; fibroblasts; Fibroblasts - drug effects; Fibroblasts - metabolism; Food Irradiation; high performance liquid chromatography; irradiation; isomerization; lycopene; Lycopersicon esculentum - chemistry; Lycopersicon esculentum - radiation effects; manufacturing; meat products; mitogen-activated protein kinase; oxidative stress; Plant Extracts - chemistry; Plant Extracts - pharmacology; Powders - chemistry; Powders - radiation effects; proteins; Rats; reactive oxygen species; Reactive Oxygen Species - metabolism; ready-to-eat foods; tomatoes
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf4048012

Tomato powder (TP) and dry tomato peel (DTP) have been previously used in our laboratory as a source of lycopene to manufacture meat products ready-to-eat (RTE) submitted to E-beam irradiation with good technological and sensory results. Present work describes the studies performed in order to investigate the effect of radiation on chemical changes and antioxidant properties of lycopene. DTP and TP were irradiated (4 kGy). Changes on lycopene were analyzed by HPLC; inhibition of reactive oxygen species (ROS), possible modulation of mitogen-activated protein kinases (MAPK) cascade, nuclear factor κ-light-chain-enhancer of activated B cells (NP-κB) activation and expression of proteins involved in oxidation stress were analyzed in RAT-1 fibroblasts cell culture. Radiation reduced the content of all-E-lycopene and increased (Z)-lycopene, lycopene isomerization, and degradation being higher in DTP than in TP. E-Beam treatment increased the antioxidant ability of both DTP and TP in inhibiting spontaneous and H2O2-induced oxidative stress in cultured fibroblasts. Antioxidant activity was higher in DTP than in TP samples.