Actin cytoskeleton reorganization correlates with polarization of secretory vesicle and cell morphology in the degranulation of mast cell subtypes in human colon tissues

Mast cells play a central role in the intestinal immune response. To investigate the relationship between degranulation, cell polarization and the reorganization of actin cytoskeleton of mast cells, we used fluorescence or gold labeling methods to identify different mast cell subtypes in human colon...

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Bibliographic Details
Published in:Acta histochemica 2014-03, Vol.116 (2), p.407-414
Main Authors: Lin, Jue-Long, Chen, Chun-Gui, Shen, Zhi-Zhong, Piao, Zhong-Xian, Li, Wei-Qiu, Liu, Liu, Xu, Li-Yan, Li, En-Min
Format: Article
Language:English
Subjects:
Actin Cytoskeleton - metabolism
Cell Degranulation
Cell Polarity
Colon - cytology
Colon - metabolism
Colon - ultrastructure
Filamentous actin reorganization
Flow Cytometry
Humans
Mast cell subtypes
Mast Cells - cytology
Mast Cells - metabolism
Mast Cells - ultrastructure
Microscopy, Confocal
Secretory vesicle
Secretory Vesicles - metabolism
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Summary:Mast cells play a central role in the intestinal immune response. To investigate the relationship between degranulation, cell polarization and the reorganization of actin cytoskeleton of mast cells, we used fluorescence or gold labeling methods to identify different mast cell subtypes in human colon. The reorganization of filamentous actin was visualized and then the polarization of secretory vesicles, as well as cell surfaces, was analyzed by fluorescence microscopy and electron microscopy. Our results first showed a diversity of filamentous actin assembly or disassembly within the contacting cell membrane of different mast cell subtypes. The polarization and degranulation of secretory vesicles was not only accompanied with the assembly and disassembly of filamentous actin at the cell periphery, but also with changes of cell surface polarization. Our study provides an insight into the local membranous structures and suggested correlations of cytoskeleton arrangement with the polarization of secretory vesicles and cell surface configuration during mast cell degranulation.
ISSN:0065-1281
1618-0372
DOI:10.1016/j.acthis.2013.09.004