Phytosulfokine alpha enhances microspore embryogenesis in both triticale and wheat

• Published in: Plant cell, tissue and organ culture tissue and organ culture, 2014, Vol.116 (1), p.125-130
• Main Authors: Asif, Muhammad, Eudes, François, Randhawa, Harpinder, Amundsen, Eric, Spaner, Dean
• Format: Article
• Language: English
• Subjects: Albinism; albino; Biomedical and Life Sciences; Cell culture; cell growth; coculture; Cultivars; Embryogenesis; Embryonic growth stage; Embryos; Life Sciences; Nursing; Ovaries; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant Sciences; Research Note; Triticale; Triticum aestivum; Wheat
• ISSN: 0167-6857; 1573-5044
• DOI: 10.1007/s11240-013-0379-y

Isolated microspore culture (IMC) has been used to develop doubled haploid lines and to generate homozygous lines in a single generation for varietal development. Phytosulfokine has been previously used in promoting cell growth and embryo development in various systems. In this study, phytosulfokine alpha (PSK-α) supplemented IMC induction medium was evaluated in triticale and wheat cultivars, and the production of embryo-like structures (ELS), green and albino plants were recorded. In addition, the contribution of ovary co-culture was also evaluated in IMC on a NPB99 + 10F induction medium. Over a range of concentrations up to 10⁻⁷M, PSK-α yielded more ELS and green plants in wheat and triticale cultivars at the highest dose, when compared to the control. It also minimized albinism in wheat cultivars, but not in triticale. Interestingly, 10⁻⁷M PSK-α also supported the formation of a large number of embryos and a few green plants in the absence of nursing ovaries.