Engineered heterologous FPP synthases-mediated Z,E-FPP synthesis in E. coli

Production of Z-type farnesyl diphosphate (FPP) has not been reported in Escherichia coli. Here we present the fusion enzyme (ILRv) of E. coli E,E-FPP synthase (IspA) and Mycobacterium tuberculosis Z,E-FPP synthase (Rv1086), which can produce primarily Z,E-FPP rather than E,E-FPP, the predominant st...

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Bibliographic Details
Published in:Metabolic engineering 2013-07, Vol.18, p.53-59
Main Authors: Wang, Chonglong, Zhou, Jia, Jang, Hui-Jeong, Yoon, Sang-Hwal, Kim, Jae-Yean, Lee, Seung-Goo, Choi, Eui-Sung, Kim, Seon-Won
Format: Article
Language:English
Subjects:
Bacterial Proteins - biosynthesis
Bacterial Proteins - genetics
Escherichia coli
Escherichia coli - enzymology
Escherichia coli - genetics
Escherichia coli - growth & development
Farnesol - metabolism
FPP synthase
Geranyltranstransferase - biosynthesis
Geranyltranstransferase - genetics
Mevalonic Acid - metabolism
Mycobacterium tuberculosis
Mycobacterium tuberculosis - enzymology
Mycobacterium tuberculosis - genetics
Polyisoprenyl Phosphates - biosynthesis
Protein fusion
Recombinant Fusion Proteins - biosynthesis
Recombinant Fusion Proteins - genetics
Sesquiterpene
Sesquiterpenes
Z,E-Farnesol
Z,E-FPP
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Summary:Production of Z-type farnesyl diphosphate (FPP) has not been reported in Escherichia coli. Here we present the fusion enzyme (ILRv) of E. coli E,E-FPP synthase (IspA) and Mycobacterium tuberculosis Z,E-FPP synthase (Rv1086), which can produce primarily Z,E-FPP rather than E,E-FPP, the predominant stereoisomer found in most organisms. Z,E-farnesol (FOH) was produced from E. coli harboring the bottom portion of the MVA pathway and the fusion FPP synthase (ILRv) at a titer of 115.6mg/L in 2YT medium containing 1% (v/v) glycerol as a carbon source and 5mM mevalonate. The Z,E-FOH production was improved by 15-fold, compared with 7.7mg/L obtained from the co-overexpression of separate IspA and Rv1086. The Z,E-FPP was not metabolized in native metabolic pathways of E. coli. It would be of interest to produce Z,E-FPP derived sesquiterpenes from recombinant E. coli due to no loss of Z,E-FPP substrate in endogenous metabolism of the host strain. •Fusion of IspA and Rv1086 can primarily produce Z,E-FPP in E. coli.•Overproduction of Z,E-FPP leads to Z,E-FOH formation in E. coli.•Z,E-FPP is not metabolized for synthesis of essential isoprenoids in E. coli.
ISSN:1096-7176
1096-7184
DOI:10.1016/j.ymben.2013.04.002