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Morphological and polyamine content changes in embryogenic and non-embryogenic callus of sugarcane
Differences in competence acquisition and subsequent embryo maturation in embryogenic and non-embryogenic callus of sugarcane var. SP79-1011 were evaluated using histomorphological analysis, growth curves, numbers of somatic embryos, and polyamine contents. Embryogenic callus was formed by cells wit...
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Published in: | Plant cell, tissue and organ culture tissue and organ culture, 2013-09, Vol.114 (3), p.351-364 |
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container_start_page | 351 |
container_title | Plant cell, tissue and organ culture |
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creator | Silveira, Vanildo de Vita, Aline Martins Macedo, Amanda Ferreira Dias, Maria Fernanda Ribeiro Floh, Eny Iochevet Segal Santa-Catarina, Claudete |
description | Differences in competence acquisition and subsequent embryo maturation in embryogenic and non-embryogenic callus of sugarcane var. SP79-1011 were evaluated using histomorphological analysis, growth curves, numbers of somatic embryos, and polyamine contents. Embryogenic callus was formed by cells with embryogenic characteristics such as a rounded shape, prominent nuclei, a high nucleus: cytoplasm ratio, small vacuoles and organized globular structures. However, non-embryogenic callus presented dispersed, elongated and vacuolated cells with a low nucleus: cytoplasm ratio; these characteristics did not allow for the development of somatic embryos even upon exposure to a maturation stimulus. These results suggest that non-embryogenic callus does not acquire embryogenic competence during induction and that maturation treatment is not sufficient to promote somatic embryo differentiation. The use of activated charcoal (AC; 1.5 g L
−1
) resulted in a higher somatic embryo maturation rate in embryogenic callus but did not yield success in non-embryogenic callus. Embryogenic callus incubated with control (10 μM 2,4-dichlorophenoxyacetic acid) and maturation (1.5 g L
−1
AC) treatments for 28 days showed similar patterns of total free polyamines; these results differed from the results observed with non-embryogenic callus, suggesting that embryogenic callus already exhibits a characteristic pattern of endogenous polyamine levels. At 28 days of culture with maturation treatment, embryogenic callus exhibited significantly higher levels of free Spm than embryogenic callus incubated with control treatment and non-embryogenic callus incubated with both treatments. This result suggests that Spm could be important for the acquisition of embryogenic competence and somatic embryo maturation in sugarcane var. SP79-1011. |
doi_str_mv | 10.1007/s11240-013-0330-2 |
format | article |
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−1
) resulted in a higher somatic embryo maturation rate in embryogenic callus but did not yield success in non-embryogenic callus. Embryogenic callus incubated with control (10 μM 2,4-dichlorophenoxyacetic acid) and maturation (1.5 g L
−1
AC) treatments for 28 days showed similar patterns of total free polyamines; these results differed from the results observed with non-embryogenic callus, suggesting that embryogenic callus already exhibits a characteristic pattern of endogenous polyamine levels. At 28 days of culture with maturation treatment, embryogenic callus exhibited significantly higher levels of free Spm than embryogenic callus incubated with control treatment and non-embryogenic callus incubated with both treatments. This result suggests that Spm could be important for the acquisition of embryogenic competence and somatic embryo maturation in sugarcane var. SP79-1011.</description><identifier>ISSN: 0167-6857</identifier><identifier>EISSN: 1573-5044</identifier><identifier>DOI: 10.1007/s11240-013-0330-2</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Activated carbon ; Activated charcoal ; Adenylate cyclase ; Biomedical and Life Sciences ; Callus ; Cell culture ; Charcoal ; Cytoplasm ; Dichlorophenoxyacetic acid ; Elongated structure ; Embryos ; Growth curves ; Life Sciences ; Maturation ; Nuclei ; Nuclei (cytology) ; Original Paper ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Polyamines ; Somatic embryos ; Sugarcane ; Vacuoles</subject><ispartof>Plant cell, tissue and organ culture, 2013-09, Vol.114 (3), p.351-364</ispartof><rights>Springer Science+Business Media Dordrecht 2013</rights><rights>Plant Cell, Tissue and Organ Culture (PCTOC) is a copyright of Springer, (2013). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c349t-97de633e82850e1eb52708eb9801aca098fb817db18200f4ac32d99099d05bf03</citedby><cites>FETCH-LOGICAL-c349t-97de633e82850e1eb52708eb9801aca098fb817db18200f4ac32d99099d05bf03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Silveira, Vanildo</creatorcontrib><creatorcontrib>de Vita, Aline Martins</creatorcontrib><creatorcontrib>Macedo, Amanda Ferreira</creatorcontrib><creatorcontrib>Dias, Maria Fernanda Ribeiro</creatorcontrib><creatorcontrib>Floh, Eny Iochevet Segal</creatorcontrib><creatorcontrib>Santa-Catarina, Claudete</creatorcontrib><title>Morphological and polyamine content changes in embryogenic and non-embryogenic callus of sugarcane</title><title>Plant cell, tissue and organ culture</title><addtitle>Plant Cell Tiss Organ Cult</addtitle><description>Differences in competence acquisition and subsequent embryo maturation in embryogenic and non-embryogenic callus of sugarcane var. SP79-1011 were evaluated using histomorphological analysis, growth curves, numbers of somatic embryos, and polyamine contents. Embryogenic callus was formed by cells with embryogenic characteristics such as a rounded shape, prominent nuclei, a high nucleus: cytoplasm ratio, small vacuoles and organized globular structures. However, non-embryogenic callus presented dispersed, elongated and vacuolated cells with a low nucleus: cytoplasm ratio; these characteristics did not allow for the development of somatic embryos even upon exposure to a maturation stimulus. These results suggest that non-embryogenic callus does not acquire embryogenic competence during induction and that maturation treatment is not sufficient to promote somatic embryo differentiation. The use of activated charcoal (AC; 1.5 g L
−1
) resulted in a higher somatic embryo maturation rate in embryogenic callus but did not yield success in non-embryogenic callus. Embryogenic callus incubated with control (10 μM 2,4-dichlorophenoxyacetic acid) and maturation (1.5 g L
−1
AC) treatments for 28 days showed similar patterns of total free polyamines; these results differed from the results observed with non-embryogenic callus, suggesting that embryogenic callus already exhibits a characteristic pattern of endogenous polyamine levels. At 28 days of culture with maturation treatment, embryogenic callus exhibited significantly higher levels of free Spm than embryogenic callus incubated with control treatment and non-embryogenic callus incubated with both treatments. This result suggests that Spm could be important for the acquisition of embryogenic competence and somatic embryo maturation in sugarcane var. SP79-1011.</description><subject>Activated carbon</subject><subject>Activated charcoal</subject><subject>Adenylate cyclase</subject><subject>Biomedical and Life Sciences</subject><subject>Callus</subject><subject>Cell culture</subject><subject>Charcoal</subject><subject>Cytoplasm</subject><subject>Dichlorophenoxyacetic acid</subject><subject>Elongated structure</subject><subject>Embryos</subject><subject>Growth curves</subject><subject>Life Sciences</subject><subject>Maturation</subject><subject>Nuclei</subject><subject>Nuclei (cytology)</subject><subject>Original Paper</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Polyamines</subject><subject>Somatic embryos</subject><subject>Sugarcane</subject><subject>Vacuoles</subject><issn>0167-6857</issn><issn>1573-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNp1kE1LxDAURYMoOI7-AHcFN26iL0nTJksZ_IIRN7oOaZp2OrRJTdrF_HszjqAIrh48zr1cDkKXBG4IQHkbCaE5YCAMA2OA6RFaEF4yzCHPj9ECSFHiQvDyFJ3FuAWAguVkgaoXH8aN733bGd1n2tXZ6PudHjpnM-PdZN2UmY12rY1Z5zI7VGHnW-s68wU77_DvXyrp55j5Jotzq4PRzp6jk0b30V583yV6f7h_Wz3h9evj8-pujQ3L5YRlWduCMSuo4GCJrTgtQdhKCiDaaJCiqQQp64oICtDk2jBaSwlS1sCrBtgSXR96x-A_ZhsnNXTR2L5PG_wcFeFJVCGp5Am9-oNu_RxcWqco5ZIJASATRQ6UCT7GYBs1hm7QYacIqL11dbCuknW1t65oytBDJiY2SQs_zf-HPgGuQYTN</recordid><startdate>20130901</startdate><enddate>20130901</enddate><creator>Silveira, Vanildo</creator><creator>de Vita, Aline Martins</creator><creator>Macedo, Amanda Ferreira</creator><creator>Dias, Maria Fernanda Ribeiro</creator><creator>Floh, Eny Iochevet Segal</creator><creator>Santa-Catarina, Claudete</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20130901</creationdate><title>Morphological and polyamine content changes in embryogenic and non-embryogenic callus of sugarcane</title><author>Silveira, Vanildo ; de Vita, Aline Martins ; Macedo, Amanda Ferreira ; Dias, Maria Fernanda Ribeiro ; Floh, Eny Iochevet Segal ; Santa-Catarina, Claudete</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c349t-97de633e82850e1eb52708eb9801aca098fb817db18200f4ac32d99099d05bf03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Activated carbon</topic><topic>Activated charcoal</topic><topic>Adenylate cyclase</topic><topic>Biomedical and Life Sciences</topic><topic>Callus</topic><topic>Cell culture</topic><topic>Charcoal</topic><topic>Cytoplasm</topic><topic>Dichlorophenoxyacetic acid</topic><topic>Elongated structure</topic><topic>Embryos</topic><topic>Growth curves</topic><topic>Life Sciences</topic><topic>Maturation</topic><topic>Nuclei</topic><topic>Nuclei (cytology)</topic><topic>Original Paper</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Polyamines</topic><topic>Somatic embryos</topic><topic>Sugarcane</topic><topic>Vacuoles</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Silveira, Vanildo</creatorcontrib><creatorcontrib>de Vita, Aline Martins</creatorcontrib><creatorcontrib>Macedo, Amanda Ferreira</creatorcontrib><creatorcontrib>Dias, Maria Fernanda Ribeiro</creatorcontrib><creatorcontrib>Floh, Eny Iochevet Segal</creatorcontrib><creatorcontrib>Santa-Catarina, Claudete</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>Biological Sciences</collection><collection>Agriculture Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Plant cell, tissue and organ culture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Silveira, Vanildo</au><au>de Vita, Aline Martins</au><au>Macedo, Amanda Ferreira</au><au>Dias, Maria Fernanda Ribeiro</au><au>Floh, Eny Iochevet Segal</au><au>Santa-Catarina, Claudete</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Morphological and polyamine content changes in embryogenic and non-embryogenic callus of sugarcane</atitle><jtitle>Plant cell, tissue and organ culture</jtitle><stitle>Plant Cell Tiss Organ Cult</stitle><date>2013-09-01</date><risdate>2013</risdate><volume>114</volume><issue>3</issue><spage>351</spage><epage>364</epage><pages>351-364</pages><issn>0167-6857</issn><eissn>1573-5044</eissn><abstract>Differences in competence acquisition and subsequent embryo maturation in embryogenic and non-embryogenic callus of sugarcane var. SP79-1011 were evaluated using histomorphological analysis, growth curves, numbers of somatic embryos, and polyamine contents. Embryogenic callus was formed by cells with embryogenic characteristics such as a rounded shape, prominent nuclei, a high nucleus: cytoplasm ratio, small vacuoles and organized globular structures. However, non-embryogenic callus presented dispersed, elongated and vacuolated cells with a low nucleus: cytoplasm ratio; these characteristics did not allow for the development of somatic embryos even upon exposure to a maturation stimulus. These results suggest that non-embryogenic callus does not acquire embryogenic competence during induction and that maturation treatment is not sufficient to promote somatic embryo differentiation. The use of activated charcoal (AC; 1.5 g L
−1
) resulted in a higher somatic embryo maturation rate in embryogenic callus but did not yield success in non-embryogenic callus. Embryogenic callus incubated with control (10 μM 2,4-dichlorophenoxyacetic acid) and maturation (1.5 g L
−1
AC) treatments for 28 days showed similar patterns of total free polyamines; these results differed from the results observed with non-embryogenic callus, suggesting that embryogenic callus already exhibits a characteristic pattern of endogenous polyamine levels. At 28 days of culture with maturation treatment, embryogenic callus exhibited significantly higher levels of free Spm than embryogenic callus incubated with control treatment and non-embryogenic callus incubated with both treatments. This result suggests that Spm could be important for the acquisition of embryogenic competence and somatic embryo maturation in sugarcane var. SP79-1011.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s11240-013-0330-2</doi><tpages>14</tpages></addata></record> |
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subjects | Activated carbon Activated charcoal Adenylate cyclase Biomedical and Life Sciences Callus Cell culture Charcoal Cytoplasm Dichlorophenoxyacetic acid Elongated structure Embryos Growth curves Life Sciences Maturation Nuclei Nuclei (cytology) Original Paper Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences Polyamines Somatic embryos Sugarcane Vacuoles |
title | Morphological and polyamine content changes in embryogenic and non-embryogenic callus of sugarcane |
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