Differential salt-stress response during germination and vegetative growth in in vitro selected somaclonal mutants of Cenchrus ciliaris L

• Published in: South African journal of botany 2013-07, Vol.87, p.157-163
• Main Authors: López Colomba, E., Tommasino, E., Luna, C., Griffa, S., Carloni, E., Ribotta, A., Quiroga, M., Grunberg, K.
• Format: Article
• Language: English
• Subjects: breeding; catalase; Cenchrus ciliaris; cultivars; developmental stages; enzyme activity; genotype; Germination; mutagenesis; mutants; salinity; Salt stress; salt tolerance; seeds; Somaclonal mutants; somaclonal variation; Vegetative growth
• ISSN: 0254-6299; 1727-9321
• DOI: 10.1016/j.sajb.2013.03.008

Four somaclonal mutants (S1, S4, S6 and M10) and their parental Cenchrus ciliaris L. cultivar Biloela were characterized under salinity conditions at germination and vegetative growth stages. Seeds of all somaclonal mutants had higher germination percentages than cv. Biloela seeds in the control and salt treatments. At 150mM, germination was significantly higher in M10, S6 and S4 (72.3%, 66.3% and 61.8%, respectively) than in cv. Biloela (35.5%). Mutants grown under salinity along with cv. Biloela for 35days had a different relative growth rate. S6 had the highest growth rate, indicating its potential tolerance to salt stress, whereas M10 was the most sensitive, with Bi, S4 and S1 being intermediate tolerant genotypes. Catalase enzyme activity (CAT) in M10 decreased in response to salt stress and was significantly associated with malondialdehide content, suggesting salt injury, whereas higher levels of CAT activity in S6 during salt stress were associated with increased salinity tolerance. The present results indicate that somaclonal variation and in vitro mutagenesis offer an effective tool for improvement of C. ciliaris because the somaclonal mutants showed differential tolerance to salt stress with respect to their parental and could be a better choice for use in a breeding program. •Somaclonal mutants were evaluated under salt stress at germination and early growth.•Somaclonal mutants had higher germination percentages than their parental.•Increasing CAT activity during salt stress was associated with salinity tolerance.•Somaclonal mutants with differential response to salinity were found.•Tissue culture and mutagenesis offer a useful tool for breeding in Cenchrus ciliaris.