Longitudinal assessment of white matter pathology in the injured mouse spinal cord through ultra-high field (16.4T) in vivo diffusion tensor imaging

• Published in: NeuroImage (Orlando, Fla.) Fla.), 2013-11, Vol.82, p.574-585
• Main Authors: Brennan, Faith H., Cowin, Gary J., Kurniawan, Nyoman D., Ruitenberg, Marc J.
• Format: Article
• Language: English
• Subjects: Animals; Anisotropy; Biological and medical sciences; Contusions; Demyelination; Diffusion Tensor Imaging - methods; Female; Fundamental and applied biological sciences. Psychology; Magnetic resonance imaging; Mice; Mice, Inbred C57BL; Nerve Fibers, Myelinated - pathology; Neurotrauma; Pathology; Performance evaluation; Spinal cord injuries; Spinal Cord Injuries - pathology; Spinal cord injury; Tractography; Vertebrates: nervous system and sense organs; Wallerian degeneration
• ISSN: 1053-8119; 1095-9572
• DOI: 10.1016/j.neuroimage.2013.06.019

This study examined the sensitivity of ultra-high field (16.4T) diffusion tensor imaging (DTI; 70μm in-plane resolution, 1mm slice thickness) to evaluate the spatiotemporal development of severe mid-thoracic contusive spinal cord injury (SCI) in mice. In vivo imaging was performed prior to SCI, then again at 2h, 1day, 3days, 7days, and 30days post-SCI using a Bruker 16.4T small animal nuclear magnetic resonance spectrometer. Cross-sectional spinal cord areas were measured in axial slices and various DTI parameters, i.e. fractional anisotropy (FA), mean diffusivity (MD), axial diffusivity (λ||) and radial diffusivity (λ⊥), were calculated for the total spared white matter (WM), ventral funiculi (VF), lateral funiculi (LF) and dorsal columns (DCs) and then correlated with histopathology. Cross-sectional area measurements revealed significant atrophy (32% reduction) of the injured spinal cord at the lesion epicentre in the chronic phase of injury. Analysis of diffusion tensor parameters further showed that tissue integrity was most severely affected in the DCs, i.e. the site of immediate impact, which demonstrated a rapid and permanent decrease in FA and λ||. In contrast, DTI parameters for the ventrolateral white matter changed more gradually with time, suggesting that these regions are undergoing more delayed degeneration in a manner that may be amenable to therapeutic intervention. Of all the DTI parameters, λ⊥ was most closely correlated to myelin content whereas changes in FA and λ|| appeared more indicative of axonal integrity, Wallerian degeneration and associated presence of macrophages. We conclude that longitudinal DTI at 16.4T provides a clinically relevant, objective measure for assessing white matter pathology following contusive SCI in mice that may aid the translation of putative neuroprotective strategies into the clinic. •DTI accurately detects white matter pathology in live mice with spinal cord injury.•Significant atrophy of the spinal cord occurs between 7 and 30days post-injury.•Spatiotemporal changes in FA and λ|| link to axon damage and Wallerian degeneration.•Increased λ⊥ correlates with injury-induced demyelination and astrogliosis.•Tractography can show white matter integrity in mice with spinal cord injury.