The production of 1,3-propanediol from mixtures of glycerol and glucose by a Klebsiella pneumoniae mutant deficient in carbon catabolite repression

► We prepared a crr mutant strain of Klebsiella pneumonia mutant, in which carbon catabolite repression (CCR) was abolished. ► Production of 1,3-propanediol from glycerol upon fermentation of the crr mutant increased by the presence of glucose. ► This is the first report to show that elimination of...

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Bibliographic Details
Published in:Bioresource technology 2013-02, Vol.130, p.719-724
Main Authors: Oh, Baek-Rock, Hong, Won-Kyung, Heo, Sun-Yeon, Luo, Lian Hua, Kondo, Akihiko, Seo, Jeong-Woo, Kim, Chul Ho
Format: Article
Language:English
Subjects:
1,3-Propanediol
Bacterial Proteins - genetics
Biological and medical sciences
Carbon
Carbon catabolite repression
Catabolite Repression - genetics
crr
Deletion
Fermentation
Fundamental and applied biological sciences. Psychology
Genes
Glucose
Glucose - metabolism
Glycerol - metabolism
Glycerols
Klebsiella
Klebsiella pneumoniae
Klebsiella pneumoniae - genetics
Klebsiella pneumoniae - metabolism
Mixtures of glycerol and glucose
Molasses
Mutation
Optimization
Phosphoenolpyruvate Sugar Phosphotransferase System - genetics
Propylene Glycols - metabolism
Strain
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Summary:► We prepared a crr mutant strain of Klebsiella pneumonia mutant, in which carbon catabolite repression (CCR) was abolished. ► Production of 1,3-propanediol from glycerol upon fermentation of the crr mutant increased by the presence of glucose. ► This is the first report to show that elimination of CCR in K. pneumoniae strains used for 1,3-PD biosynthesis. In the present study, mutant strain of Klebsiella pneumoniae with deletion of the crr gene encoding EIIAGlc (a component of the glucose-specific phosphoenolpyruvate-dependent transferase system [PTS]) was prepared. This eliminated the ability of the strain to mediate carbon catabolite repression (CCR). Production of 1,3-propanediol (1,3-PD) from glycerol by the crr mutant strain was enhanced (compared to that of the parent) in the presence of glucose. Using molasses as a co-substrate of glycerol, the maximum yield of 1,3-PD was 60.4% greater (81.2g/l) than that obtained when glycerol was used alone, under optimum fermentation conditions.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2012.12.076