Catabolism of 4-alkylphenols by Acinetobacter sp. OP5: Genetic organization of the oph gene cluster and characterization of alkylcatechol 2, 3-dioxygenase

► An effective technique to detect the estrogen-like alkylphenol degradation gene cluster was first reported. ► The first study of the genes and enzyme in the degradation of octylphenol from Acinetobacter sp. ► Evidence of the aromatic ring cleavage of 4-t-alkylcatechol is demonstrated for the first...

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Bibliographic Details
Published in:Bioresource technology 2013-03, Vol.131, p.420-428
Main Authors: Tuan, Nguyen Ngoc, Lin, Yi-Wen, Huang, Shir-Ly
Format: Article
Language:English
Subjects:
Acinetobacter - enzymology
Acinetobacter - genetics
Acinetobacter - isolation & purification
Alkylcatechol 2, 3-dioxygenase
Alkylphenol
Bacteria
Biodegradation, Environmental
Biological and medical sciences
Biomarkers
Catabolism
Catechol 2,3-Dioxygenase - chemistry
Catechol 2,3-Dioxygenase - genetics
Catechol 2,3-Dioxygenase - metabolism
Clusters
Fundamental and applied biological sciences. Psychology
Genes
Genetics
Metabolism
Multigene Family - genetics
Phenol hydroxygenase
Phenols - isolation & purification
Phenols - metabolism
Recombinant
Strain
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Summary:► An effective technique to detect the estrogen-like alkylphenol degradation gene cluster was first reported. ► The first study of the genes and enzyme in the degradation of octylphenol from Acinetobacter sp. ► Evidence of the aromatic ring cleavage of 4-t-alkylcatechol is demonstrated for the first time. In this study, a specific PCR primer set was successfully designed for alkylcatechol 2, 3-dioxygenase genes and applied to detect the presence of this biomarker in 4-t-octylphenol-degrading Acinetobacter sp. strain OP5. A gene cluster (ophRBA1A2A3A4A5A6CEH) encoding multicomponent phenol hydroxylase and alkylcatechol 2, 3-dioxygenase was then cloned from this strain and showed the highest homology to those involved in the published medium-chain alkylphenol gene clusters. The pure enzyme of recombinant cell harboring ophB showed meta-cleavage activities for 4-methylcatechol (1,435%), 4-ethylcatechol (982%), catechol (100%), 4-t-butylcatechol (16.6%), and 4-t-octylcatechol (3.2%). The results suggest that the developed molecular technique is useful and easy in detection of medium/long-chain alkylphenol degradation gene cluster. In addition, it also provides a better understanding of the distribution of biodegradative genes and pathway for estrogenic-active long-chain alkylphenols in bacteria.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2012.12.086