The immune-adjuvant effect of Japanese flounder Paralichthys olivaceus IL-1I2

IL-1I2 is known as a pro-inflammatory cytokine and plays a pivotal role in regulating immune response. IL-1I2 has been shown to influence immune responses in Japanese flounder Paralichthys olivaceus. To investigate the immune responses, a plasmid construct of pcDNA3.1-driven Japanese flounder IL-1I2...

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Bibliographic Details
Published in:Developmental and comparative immunology 2013-08, Vol.40 (3-4), p.564-568
Main Authors: Taechavasonyoo, Apichaya, Hirono, Ikuo, Kondo, Hidehiro
Format: Article
Language:English
Subjects:
Paralichthys olivaceus
Pleuronectiformes
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Summary:IL-1I2 is known as a pro-inflammatory cytokine and plays a pivotal role in regulating immune response. IL-1I2 has been shown to influence immune responses in Japanese flounder Paralichthys olivaceus. To investigate the immune responses, a plasmid construct of pcDNA3.1-driven Japanese flounder IL-1I2 (pcDNA3.1-JFIL-1I2) was co-injected into the muscle with Bovine serum albumin (BSA), as an antigen model, or pCI-neo driven with GFP (pCI-neo-GFP) as a vaccine model compared with the antigen or vaccine model alone, respectively. The IL-1I2 expression in the muscle was dramatically elevated in fish injected with pcDNA3.1-JFIL-1I2 on a day after injection, and the induction level was significantly higher than control groups. Moreover, pcDNA3.1-JFIL-1I2 significantly stimulated the gene expression of IL-1I2 in the kidney. The pcDNA3.1-JFIL-1I2 enhanced the antibody titer against BSA at 30 days after injection. In the DNA vaccine model, the antibody titer against GFP was also higher in the fish injected with pcDNA3.1-JFIL-1I2 than the group that injected pCI-neo-GFP alone. These results suggest that the pcDNA-driven Japanese flounder IL-1I2 could have potential immunoadjuvant effects.
ISSN:0145-305X
DOI:10.1016/j.dci.2013.07.003