Dynamic changes in viral population structure and compartmentalization during chronic hepatitis C virus infection in children

Abstract Classic phylogenetic and modern population-based clustering methods were used to analyze hepatitis C virus (HCV) evolution in plasma and to assess viral compartmentalization within peripheral blood mononuclear cells (PBMCs) in 6 children during 3.2–9.6 yr of follow-up. Population structure...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2013-12, Vol.447 (1), p.187-196
Main Authors: Gismondi, María Inés, Díaz Carrasco, Juan María, Valva, Pamela, Becker, Pablo Daniel, Guzmán, Carlos Alberto, Campos, Rodolfo Héctor, Preciado, María Victoria
Format: Article
Language:English
Subjects:
Adolescent
Biota
Child
Child, Preschool
Cluster Analysis
Compartmentalization
Evolution
Female
Genetic Variation
Hepacivirus - classification
Hepacivirus - genetics
Hepacivirus - isolation & purification
Hepatitis C virus
Hepatitis C, Chronic - virology
Humans
Infectious Disease
Leukocytes, Mononuclear - virology
Male
Molecular Sequence Data
PBMC
Pediatric infection
Phylogeny
Plasma - virology
Population structure
Retrospective Studies
RNA, Viral - genetics
Sequence Analysis, DNA
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Summary:Abstract Classic phylogenetic and modern population-based clustering methods were used to analyze hepatitis C virus (HCV) evolution in plasma and to assess viral compartmentalization within peripheral blood mononuclear cells (PBMCs) in 6 children during 3.2–9.6 yr of follow-up. Population structure analysis of cloned amplicons encompassing hypervariable region 1 led to the distinction of two evolutionary patterns, one highly divergent and another one genetically homogeneous. Viral adaptability was reflected by co-evolution of viral communities switching rapidly from one to another in the context of divergence and stability associated with highly homogeneous communities which were replaced by new ones after long periods. Additionally, viral compartmentalization of HCV in PBMCs was statistically demonstrated, suggesting their role as a pool of genetic variability. Our results support the idea of a community-based structure of HCV viral populations during chronic infection and highlight a role of the PBMC compartment in the persistence of such structure.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2013.09.002