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Induction of humoral and cellular immune responses by antigen-expressing immunostimulatory liposomes
Recently we have shown that liposomes can be used as artificial microbes for the production and delivery of DNA-encoded antigens. These so-called antigen-expressing immunostimulatory liposomes (AnExILs) were superior in inducing antigen-specific antibodies compared to conventional liposomal protein...
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Published in: | Journal of controlled release 2012-12, Vol.164 (3), p.323-330 |
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creator | Amidi, Maryam van Helden, Mary J.G. Tabataei, Neda Rafiei de Goede, Anna L. Schouten, Marijn de Bot, Volkert Lanzi, Anastasia Gruters, Rob A. Rimmelzwaan, Guus F. Sijts, Alice J.A.M. Mastrobattista, Enrico |
description | Recently we have shown that liposomes can be used as artificial microbes for the production and delivery of DNA-encoded antigens. These so-called antigen-expressing immunostimulatory liposomes (AnExILs) were superior in inducing antigen-specific antibodies compared to conventional liposomal protein or DNA vaccines when tested in mice after i.m. immunization. In this study, we investigated the capacity of AnExILs to induce T-cell responses. By using a plasmid vector encoding a model antigen under control of both the prokaryotic T7 and the eukaryotic CMV promoter we hypothesized that antigen production could lead to CTL activation via two distinct routes: i. production of antigens inside the AnExILs with subsequent cross-presentation after processing by APCs and ii. endogenous production of antigens after AnExIL-mediated transfection of the pDNA. Although we were not able to demonstrate transfection-mediated expression of luc-NP in mice, i.m. injection of AnExILs producing luc-NP resulted in T-cell responses against the encoded NP epitope, as determined by tetramer staining. T-cell responses were comparable to the responses obtained after i.m. injection of naked pDNA. In order to find out whether CTL activation was caused by cross-presentation of the exogenous antigens produced inside AnExILs or by endogenous antigen production from transfection with the same pDNA source a second study was initiated in which the contribution of each of these effects could be separately determined. These results demonstrate that the observed T-cell responses were not exclusively caused by cross-presentation of the AnExIL-produced antigens alone, but were rather a combination of dose-dependent antigen cross-presentation and low levels of endogenous antigen production.
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doi_str_mv | 10.1016/j.jconrel.2012.08.016 |
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[Display omitted]</description><identifier>ISSN: 0168-3659</identifier><identifier>EISSN: 1873-4995</identifier><identifier>DOI: 10.1016/j.jconrel.2012.08.016</identifier><identifier>PMID: 22940204</identifier><identifier>CODEN: JCREEC</identifier><language>eng</language><publisher>Kidlington: Elsevier B.V</publisher><subject>Adjuvants, Immunologic - administration & dosage ; Animals ; antibodies ; Antigens - genetics ; Antigens - immunology ; beta-Galactosidase - genetics ; beta-Galactosidase - immunology ; Biological and medical sciences ; Cell-free protein synthesis ; Cell-mediated immunity ; Cytomegalovirus ; DNA vaccines ; dose response ; epitopes ; Female ; General pharmacology ; Immunity, Cellular - immunology ; Immunity, Humoral - immunology ; immunization ; Liposomes ; Luciferases - genetics ; Luciferases - immunology ; Lymphocyte Activation - immunology ; Medical sciences ; Mice ; Mice, Inbred C57BL ; microorganisms ; Pharmaceutical technology. Pharmaceutical industry ; Pharmacology. Drug treatments ; plasmid vectors ; Plasmids ; recombinant vaccines ; T-lymphocytes ; T-Lymphocytes - immunology ; transfection ; Vaccines, DNA - administration & dosage ; Vaccines, DNA - immunology</subject><ispartof>Journal of controlled release, 2012-12, Vol.164 (3), p.323-330</ispartof><rights>2012 Elsevier B.V.</rights><rights>2014 INIST-CNRS</rights><rights>Copyright © 2012 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-aa80db605136898d4c0ab94fc134abd49d74b56c9180c4bfee5ad9a20389c9e93</citedby><cites>FETCH-LOGICAL-c452t-aa80db605136898d4c0ab94fc134abd49d74b56c9180c4bfee5ad9a20389c9e93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26919653$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22940204$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Amidi, Maryam</creatorcontrib><creatorcontrib>van Helden, Mary J.G.</creatorcontrib><creatorcontrib>Tabataei, Neda Rafiei</creatorcontrib><creatorcontrib>de Goede, Anna L.</creatorcontrib><creatorcontrib>Schouten, Marijn</creatorcontrib><creatorcontrib>de Bot, Volkert</creatorcontrib><creatorcontrib>Lanzi, Anastasia</creatorcontrib><creatorcontrib>Gruters, Rob A.</creatorcontrib><creatorcontrib>Rimmelzwaan, Guus F.</creatorcontrib><creatorcontrib>Sijts, Alice J.A.M.</creatorcontrib><creatorcontrib>Mastrobattista, Enrico</creatorcontrib><title>Induction of humoral and cellular immune responses by antigen-expressing immunostimulatory liposomes</title><title>Journal of controlled release</title><addtitle>J Control Release</addtitle><description>Recently we have shown that liposomes can be used as artificial microbes for the production and delivery of DNA-encoded antigens. These so-called antigen-expressing immunostimulatory liposomes (AnExILs) were superior in inducing antigen-specific antibodies compared to conventional liposomal protein or DNA vaccines when tested in mice after i.m. immunization. In this study, we investigated the capacity of AnExILs to induce T-cell responses. By using a plasmid vector encoding a model antigen under control of both the prokaryotic T7 and the eukaryotic CMV promoter we hypothesized that antigen production could lead to CTL activation via two distinct routes: i. production of antigens inside the AnExILs with subsequent cross-presentation after processing by APCs and ii. endogenous production of antigens after AnExIL-mediated transfection of the pDNA. Although we were not able to demonstrate transfection-mediated expression of luc-NP in mice, i.m. injection of AnExILs producing luc-NP resulted in T-cell responses against the encoded NP epitope, as determined by tetramer staining. T-cell responses were comparable to the responses obtained after i.m. injection of naked pDNA. In order to find out whether CTL activation was caused by cross-presentation of the exogenous antigens produced inside AnExILs or by endogenous antigen production from transfection with the same pDNA source a second study was initiated in which the contribution of each of these effects could be separately determined. These results demonstrate that the observed T-cell responses were not exclusively caused by cross-presentation of the AnExIL-produced antigens alone, but were rather a combination of dose-dependent antigen cross-presentation and low levels of endogenous antigen production.
[Display omitted]</description><subject>Adjuvants, Immunologic - administration & dosage</subject><subject>Animals</subject><subject>antibodies</subject><subject>Antigens - genetics</subject><subject>Antigens - immunology</subject><subject>beta-Galactosidase - genetics</subject><subject>beta-Galactosidase - immunology</subject><subject>Biological and medical sciences</subject><subject>Cell-free protein synthesis</subject><subject>Cell-mediated immunity</subject><subject>Cytomegalovirus</subject><subject>DNA vaccines</subject><subject>dose response</subject><subject>epitopes</subject><subject>Female</subject><subject>General pharmacology</subject><subject>Immunity, Cellular - immunology</subject><subject>Immunity, Humoral - immunology</subject><subject>immunization</subject><subject>Liposomes</subject><subject>Luciferases - genetics</subject><subject>Luciferases - immunology</subject><subject>Lymphocyte Activation - immunology</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>microorganisms</subject><subject>Pharmaceutical technology. Pharmaceutical industry</subject><subject>Pharmacology. Drug treatments</subject><subject>plasmid vectors</subject><subject>Plasmids</subject><subject>recombinant vaccines</subject><subject>T-lymphocytes</subject><subject>T-Lymphocytes - immunology</subject><subject>transfection</subject><subject>Vaccines, DNA - administration & dosage</subject><subject>Vaccines, DNA - immunology</subject><issn>0168-3659</issn><issn>1873-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNqFkcuO1DAQRS0EYpqBTwCyQWKTUHbsxF4hNOIx0kgsYNaWY1catxK7sRNE_z1upYHlrEq6Ordel5CXFBoKtHt3aA42hoRTw4CyBmRT1EdkR2Xf1lwp8ZjsiiLrthPqijzL-QAAouX9U3LFmOLAgO-Iuw1utYuPoYpj9WOdYzJTZYKrLE7TOplU-XleA1YJ8zGGjLkaTgVY_B5Djb-PRc8-7Dcs5sXPxbXEdKomf4w5zpifkyejmTK-uNRrcv_p4_ebL_Xd18-3Nx_uassFW2pjJLihA0HbTirpuAUzKD5a2nIzOK5czwfRWUUlWD6MiMI4ZRi0UlmFqr0mb7e-xxR_rpgXPft8vsMEjGvWVAD0vehZ_zDKGIhOltYFFRtqU8w54aiPyc8mnTQFfc5CH_QlC33OQoPURS2-V5cR6zCj--f6-_wCvLkAJlszjckE6_N_rlNUdaIt3OuNG03UZp8Kc_-tTCrX0L5T_XnF9xuB5bu_PCadrcdg0fmEdtEu-geW_QM50LWo</recordid><startdate>20121228</startdate><enddate>20121228</enddate><creator>Amidi, Maryam</creator><creator>van Helden, Mary J.G.</creator><creator>Tabataei, Neda Rafiei</creator><creator>de Goede, Anna L.</creator><creator>Schouten, Marijn</creator><creator>de Bot, Volkert</creator><creator>Lanzi, Anastasia</creator><creator>Gruters, Rob A.</creator><creator>Rimmelzwaan, Guus F.</creator><creator>Sijts, Alice J.A.M.</creator><creator>Mastrobattista, Enrico</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>20121228</creationdate><title>Induction of humoral and cellular immune responses by antigen-expressing immunostimulatory liposomes</title><author>Amidi, Maryam ; van Helden, Mary J.G. ; Tabataei, Neda Rafiei ; de Goede, Anna L. ; Schouten, Marijn ; de Bot, Volkert ; Lanzi, Anastasia ; Gruters, Rob A. ; Rimmelzwaan, Guus F. ; Sijts, Alice J.A.M. ; Mastrobattista, Enrico</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-aa80db605136898d4c0ab94fc134abd49d74b56c9180c4bfee5ad9a20389c9e93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adjuvants, Immunologic - administration & dosage</topic><topic>Animals</topic><topic>antibodies</topic><topic>Antigens - genetics</topic><topic>Antigens - immunology</topic><topic>beta-Galactosidase - genetics</topic><topic>beta-Galactosidase - immunology</topic><topic>Biological and medical sciences</topic><topic>Cell-free protein synthesis</topic><topic>Cell-mediated immunity</topic><topic>Cytomegalovirus</topic><topic>DNA vaccines</topic><topic>dose response</topic><topic>epitopes</topic><topic>Female</topic><topic>General pharmacology</topic><topic>Immunity, Cellular - immunology</topic><topic>Immunity, Humoral - immunology</topic><topic>immunization</topic><topic>Liposomes</topic><topic>Luciferases - genetics</topic><topic>Luciferases - immunology</topic><topic>Lymphocyte Activation - immunology</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>microorganisms</topic><topic>Pharmaceutical technology. Pharmaceutical industry</topic><topic>Pharmacology. Drug treatments</topic><topic>plasmid vectors</topic><topic>Plasmids</topic><topic>recombinant vaccines</topic><topic>T-lymphocytes</topic><topic>T-Lymphocytes - immunology</topic><topic>transfection</topic><topic>Vaccines, DNA - administration & dosage</topic><topic>Vaccines, DNA - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Amidi, Maryam</creatorcontrib><creatorcontrib>van Helden, Mary J.G.</creatorcontrib><creatorcontrib>Tabataei, Neda Rafiei</creatorcontrib><creatorcontrib>de Goede, Anna L.</creatorcontrib><creatorcontrib>Schouten, Marijn</creatorcontrib><creatorcontrib>de Bot, Volkert</creatorcontrib><creatorcontrib>Lanzi, Anastasia</creatorcontrib><creatorcontrib>Gruters, Rob A.</creatorcontrib><creatorcontrib>Rimmelzwaan, Guus F.</creatorcontrib><creatorcontrib>Sijts, Alice J.A.M.</creatorcontrib><creatorcontrib>Mastrobattista, Enrico</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of controlled release</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Amidi, Maryam</au><au>van Helden, Mary J.G.</au><au>Tabataei, Neda Rafiei</au><au>de Goede, Anna L.</au><au>Schouten, Marijn</au><au>de Bot, Volkert</au><au>Lanzi, Anastasia</au><au>Gruters, Rob A.</au><au>Rimmelzwaan, Guus F.</au><au>Sijts, Alice J.A.M.</au><au>Mastrobattista, Enrico</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of humoral and cellular immune responses by antigen-expressing immunostimulatory liposomes</atitle><jtitle>Journal of controlled release</jtitle><addtitle>J Control Release</addtitle><date>2012-12-28</date><risdate>2012</risdate><volume>164</volume><issue>3</issue><spage>323</spage><epage>330</epage><pages>323-330</pages><issn>0168-3659</issn><eissn>1873-4995</eissn><coden>JCREEC</coden><abstract>Recently we have shown that liposomes can be used as artificial microbes for the production and delivery of DNA-encoded antigens. These so-called antigen-expressing immunostimulatory liposomes (AnExILs) were superior in inducing antigen-specific antibodies compared to conventional liposomal protein or DNA vaccines when tested in mice after i.m. immunization. In this study, we investigated the capacity of AnExILs to induce T-cell responses. By using a plasmid vector encoding a model antigen under control of both the prokaryotic T7 and the eukaryotic CMV promoter we hypothesized that antigen production could lead to CTL activation via two distinct routes: i. production of antigens inside the AnExILs with subsequent cross-presentation after processing by APCs and ii. endogenous production of antigens after AnExIL-mediated transfection of the pDNA. Although we were not able to demonstrate transfection-mediated expression of luc-NP in mice, i.m. injection of AnExILs producing luc-NP resulted in T-cell responses against the encoded NP epitope, as determined by tetramer staining. T-cell responses were comparable to the responses obtained after i.m. injection of naked pDNA. In order to find out whether CTL activation was caused by cross-presentation of the exogenous antigens produced inside AnExILs or by endogenous antigen production from transfection with the same pDNA source a second study was initiated in which the contribution of each of these effects could be separately determined. These results demonstrate that the observed T-cell responses were not exclusively caused by cross-presentation of the AnExIL-produced antigens alone, but were rather a combination of dose-dependent antigen cross-presentation and low levels of endogenous antigen production.
[Display omitted]</abstract><cop>Kidlington</cop><pub>Elsevier B.V</pub><pmid>22940204</pmid><doi>10.1016/j.jconrel.2012.08.016</doi><tpages>8</tpages></addata></record> |
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subjects | Adjuvants, Immunologic - administration & dosage Animals antibodies Antigens - genetics Antigens - immunology beta-Galactosidase - genetics beta-Galactosidase - immunology Biological and medical sciences Cell-free protein synthesis Cell-mediated immunity Cytomegalovirus DNA vaccines dose response epitopes Female General pharmacology Immunity, Cellular - immunology Immunity, Humoral - immunology immunization Liposomes Luciferases - genetics Luciferases - immunology Lymphocyte Activation - immunology Medical sciences Mice Mice, Inbred C57BL microorganisms Pharmaceutical technology. Pharmaceutical industry Pharmacology. Drug treatments plasmid vectors Plasmids recombinant vaccines T-lymphocytes T-Lymphocytes - immunology transfection Vaccines, DNA - administration & dosage Vaccines, DNA - immunology |
title | Induction of humoral and cellular immune responses by antigen-expressing immunostimulatory liposomes |
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