Optimization of the solvent extraction of phenolics and antioxidants from waste Cauliflower leaves (Brassica oleracea L.) using Response Surface Methodology (RSM)

This study investigates the extraction characteristics and optimal parameters for the solvent extraction of phenolic compounds and their antioxidants from waste cauliflower leaves. Response surface methodology by central composite design was employed to statistically optimize the extraction conditio...

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Bibliographic Details
Published in:International Journal of Biological Sciences and Technology 2013-03, Vol.5 (2), p.4-4
Main Authors: Chowdhury, Anindita, Selvaraj, K, Bhattacharjee, Chiranjib, Chowdhury, Ranjana
Format: Article
Language:English
Subjects:
Antioxidants
Brassica
Online Access:Get full text
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Summary:This study investigates the extraction characteristics and optimal parameters for the solvent extraction of phenolic compounds and their antioxidants from waste cauliflower leaves. Response surface methodology by central composite design was employed to statistically optimize the extraction conditions by varying three parameters: methanol concentration, extraction temperature and extraction time. The analysis of variance indicated that, the linear coefficients of methanol concentration and extraction temperature have significant effect on extraction of higher yield of phenolic compounds. The combined effects of independent variables were investigated and the optimal extraction conditions were obtained as methanol concentration: 75%, extraction temperature: 115.34 degree Celsius and extraction time: 72.5 min under this conditions to achieve the highest yield of total phenolic content: 485.58 mg GAE/100gm, total flavonoid content: 102.91mgQuer/100mg, and the corresponding antioxidant activities of %DPPHsc:84.77%, FRAP: 3484μgmol Fe(II)/gm and %H2O2sc: 80.85 from waste cauliflower leaves powder. The results if this validation study showed good matching. Thus, this optimization procedure could be helpful in the food and pharmaceutical industry.
ISSN:0975-8704