Inactivation of human norovirus using chemical sanitizers

The porcine gastric mucin binding magnetic bead (PGM-MB) assay was used to evaluate the ability of chlorine, chlorine dioxide, peroxyacetic acid, hydrogen peroxide, and trisodium phosphate to inactivate human norovirus within 10% stool filtrate. One-minute free chlorine treatments at concentrations...

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Bibliographic Details
Published in:International journal of food microbiology 2014-02, Vol.171, p.94-99
Main Authors: Kingsley, David H., Vincent, Emily M., Meade, Gloria K., Watson, Clytrice L., Fan, Xuetong
Format: Article
Language:English
Subjects:
Biological and medical sciences
Caliciviridae Infections - prevention & control
Chlorine
Chlorine - pharmacology
Chlorine Compounds - pharmacology
Chlorine dioxide
Disinfectants - pharmacology
Food industries
Food microbiology
Fundamental and applied biological sciences. Psychology
Human norovirus
Humans
Hydrogen peroxide
Hydrogen Peroxide - pharmacology
Norovirus
Norovirus - drug effects
Norovirus - physiology
Oxides - pharmacology
Peracetic Acid - pharmacology
Peroxyacetic acid
Phosphates - pharmacology
Sodium Hypochlorite - pharmacology
Time
Trisodium phosphate
Virus Inactivation
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Summary:The porcine gastric mucin binding magnetic bead (PGM-MB) assay was used to evaluate the ability of chlorine, chlorine dioxide, peroxyacetic acid, hydrogen peroxide, and trisodium phosphate to inactivate human norovirus within 10% stool filtrate. One-minute free chlorine treatments at concentrations of 33 and 189ppm reduced virus binding in the PGM-MB assay by 1.48 and 4.14 log10, respectively, suggesting that chlorine is an efficient sanitizer for inactivation of human norovirus (HuNoV). Five minute treatments with 5% trisodium phosphate (pH~12) reduced HuNoV binding by 1.6 log10, suggesting that TSP, or some other high pH buffer, could be used to treat food and food contact surfaces to reduce HuNoV. One minute treatments with 350ppm chlorine dioxide dissolved in water did not reduce PGM-MB binding, suggesting that the sanitizer may not be suitable for HuNoV inactivation in liquid form. However a 60-min treatment with 350ppm chlorine dioxide did reduce human norovirus by 2.8 log10, indicating that chlorine dioxide had some, albeit limited, activity against HuNoV. Results also suggest that peroxyacetic acid has limited effectiveness against human norovirus, since 1-min treatments with up to 195ppm reduced human norovirus binding by
ISSN:0168-1605
1879-3460
DOI:10.1016/j.ijfoodmicro.2013.11.018