Correlating denitrifying catabolic genes with N2O and N2 emissions from swine slurry composting

•Denitrifying catabolic genes were used to infer on N2O and N2 losses during swine slurry composting.•During the thermophilic composting stage most of the N losses occurred as NH3 and N2 emissions.•Changes in norB and nosZ genes abundance were indicative of N2O emissions.•The catabolic narG, nirS, a...

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Bibliographic Details
Published in:Bioresource technology 2013-07, Vol.140, p.368-375
Main Authors: Angnes, G., Nicoloso, R.S., da Silva, M.L.B., de Oliveira, P.A.V., Higarashi, M.M., Mezzari, M.P., Miller, P.R.M.
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Ammonia - analysis
Animal productions
Animals
Bacteria
Biological and medical sciences
Biological treatment of sewage sludges and wastes
Biotechnology
Carbon Dioxide - analysis
Composting
Correlation
Denitrification
Denitrification - genetics
Environment and pollution
Functional genes
Fundamental and applied biological sciences. Psychology
General agronomy. Plant production
Genes
Genes, Bacterial - genetics
Greenhouse gases
Humidity
Industrial applications and implications. Economical aspects
Methane - analysis
Nitrification
Nitrification - genetics
Nitrogen - analysis
Nitrous Oxide - analysis
Nitrous oxides
Piles
qPCR
Slurries
Soil
Sus scrofa
Swine
Temperature
Terrestrial animal productions
Time Factors
Use of agricultural and forest wastes. Biomass use, bioconversion
Vertebrates
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Summary:•Denitrifying catabolic genes were used to infer on N2O and N2 losses during swine slurry composting.•During the thermophilic composting stage most of the N losses occurred as NH3 and N2 emissions.•Changes in norB and nosZ genes abundance were indicative of N2O emissions.•The catabolic narG, nirS, and nosZ genes were significantly correlated with N2 emission.•Functional genes were useful to estimate N emissions and minimize uncertainties on mass balances. This work evaluated N dynamics that occurs over time within swine slurry composting piles. Real-time quantitative PCR (qPCR) analyzes were conducted to estimate concentrations of bacteria community harboring specific catabolic nitrifying-ammonium monooxygenase (amoA), and denitrifying nitrate- (narG), nitrite- (nirS and nirG), nitric oxide- (norB) and nitrous oxide reductases (nosZ) genes. NH3-N, N2O-N, N2-N emissions represented 15.4±1.9%, 5.4±0.9%, and 79.1±2.0% of the total nitrogen losses, respectively. Among the genes tested, temporal distribution of narG, nirS, and nosZ concentration correlated significantly (p
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2013.04.112