Transgenic rapeseed plants obtained by the microinjection of DNA into microspore-derived embryoids

A novel method in the field of genetic engineering of higher plants is presented: microinjection into multicellular structures which have a high competence for plant regeneration through embryogenesis. Microspore-derived embryoids of Brassica napus L. were individually selected and microinjected wit...

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Bibliographic Details
Published in:Theoretical and applied genetics 1987-01, Vol.75 (1), p.30-36
Main Authors: Neuhaus, G., Spangenberg, G., Mittelsten Scheid, O., Schweiger, H. -G.
Format: Article
Language:English
Subjects:
Brassica napus
genes
neomycin phosphotransferase
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Summary:A novel method in the field of genetic engineering of higher plants is presented: microinjection into multicellular structures which have a high competence for plant regeneration through embryogenesis. Microspore-derived embryoids of Brassica napus L. were individually selected and microinjected with NPT II gene constructions. High frequency regeneration of haploid plants through embryogenesis was achieved within 8 weeks.
ISSN:0040-5752
1432-2242
DOI:10.1007/BF00249138