Genetic diversity of yacon (Smallanthus sonchifolius (Poepp. & Endl.) H. Robinson) and its wild relatives as revealed by ISSR markers

Set of 29 accessions of Smallanthus sonchifolius, an important tuber crop from South America, and its three wild relatives were analysed using the Inter Simple Sequence Repeats (ISSR) markers. Seven primers out of 30 primers screened gave clear and reproducible spectra. The range of amplified bands...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical systematics and ecology 2013-10, Vol.50, p.383-389
Main Authors: Svobodová, Eva, Dvořáková, Zdislava, Čepková, Petra Hlásná, Viehmannová, Iva, Havlíčková, Lenka, Cusimamani, Eloy Fernández, Russo, Daniela, Meza Zela, Gregorio
Format: Article
Language:English
Subjects:
Clonal plant
genetic distance
Genetic diversity
genetic variation
ISSR
microsatellite repeats
Smallanthus
Smallanthus sonchifolius
wild relatives
Wild yacon
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Set of 29 accessions of Smallanthus sonchifolius, an important tuber crop from South America, and its three wild relatives were analysed using the Inter Simple Sequence Repeats (ISSR) markers. Seven primers out of 30 primers screened gave clear and reproducible spectra. The range of amplified bands was from 2500 bp to 300 bp. These seven primers generated in total 77 bands, from which 75 (97.4%) were polymorphic. Nei's genetic distances between samples varied from 0.01 to 0.24. The Shannon's index (I) was estimated as 0.0392. The UPGMA dendrogram created using the Neighbour joining method and based on the Dice's dissimilarity coefficient separated clearly the wild accessions from all S. sonchifolius samples, which remained close to each other, confirming the clonal origin and thus a very low genetic variability within the genus. •We have analysed Smallanthus sonchifolius accessions and its wild relatives.•We have examined them from morphological and molecular point of view.•The genetic diversity among S. sonchifolius accessions was low.•Wild plants differed significantly from the cultivated ones.
ISSN:0305-1978
1873-2925
DOI:10.1016/j.bse.2013.05.007