Hyaluronic acid / chitosan multilayer coatings on neuronal implants for localized delivery of siRNA nanoplexes

Binding, stabilizing and promoting cellular uptake of siRNA are all critical efforts in creating matrices for the localized delivery of siRNA molecules to target cells. In this study, we describe the generation of chitosan imidazole/siRNA nanoplexes (NPs) embedded in nano scope polyelectrolyte multi...

Full description

Saved in:
Bibliographic Details
Published in:Journal of controlled release 2013-06, Vol.168 (3), p.289-297
Main Authors: Hartmann, Hanna, Hossfeld, Susanne, Schlosshauer, Burkhard, Mittnacht, Ursula, Pêgo, Ana Paula, Dauner, Martin, Doser, Michael, Stoll, Dieter, Krastev, Rumen
Format: Article
Language:English
Subjects:
Animals
Cell adhesion
Cell Adhesion - drug effects
cell culture
Cell Survival - drug effects
chitosan
Chitosan - chemistry
coatings
Controlled release
drugs
electrolytes
Functionalized implants
Gene Knockdown Techniques
gene silencing
hyaluronic acid
Hyaluronic Acid - chemistry
Imidazoles - chemistry
medicine
Nanostructures - administration & dosage
Nanostructures - chemistry
Nanotherapeutics
Nerve regeneration
Neurons
PC12 Cells
Polyelectrolyte multilayers
Prostheses and Implants
Rats
rhoA GTP-Binding Protein - genetics
RNA, Small Interfering - administration & dosage
RNA, Small Interfering - chemistry
siRNA nanoplexes
small interfering RNA
spinal cord
viability
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Binding, stabilizing and promoting cellular uptake of siRNA are all critical efforts in creating matrices for the localized delivery of siRNA molecules to target cells. In this study, we describe the generation of chitosan imidazole/siRNA nanoplexes (NPs) embedded in nano scope polyelectrolyte multilayers (PEMs) composed of hyaluronic acid and chitosan for sustained and localized drug delivery. Regular PEM build-up, successful integration of NPs and controlled release under physiological conditions were shown. Biological efficacy was evaluated in neuronal cell culture concerning cell adhesion, viability, NPs uptake and gene silencing. The additionally shown biological functionalization of neuronal implants possesses potential for future applications in the field of regenerative medicine and treatment of spinal cord injuries. Chitosan Imid/siRNA nanoplexes (NPs) integrated into polyelectrolyte multilayers (PEMs) to biofunctionalize implant surfaces with novel nanotherapeutic coatings. The PEMs degrade with time and Ch Imid/siRNA NPs can be taken up by cells. [Display omitted]
ISSN:0168-3659
1873-4995
DOI:10.1016/j.jconrel.2013.03.026