High level lipid production by a novel inulinase-producing yeast Pichia guilliermondii Pcla22

• Published in: Bioresource technology 2012-11, Vol.124, p.77-82
• Main Authors: Wang, Guang-Yuan, Chi, Zhe, Song, Bo, Wang, Zhi-Peng, Chi, Zhen-Ming
• Format: Article
• Language: English
• Subjects: Biodiesel; Biodiesel production; Biofuels; Biomass; Cell Division; Consumption; Culture; Fatty acids; Fermentation; Glycoside Hydrolases - biosynthesis; Inulin; Inulin - metabolism; Inulinase; lipid bodies; Lipids; Lipids - biosynthesis; Nitrogen - metabolism; oils; P. guilliermondii; Phylogeny; Pichia; Pichia - classification; Pichia - genetics; Pichia - metabolism; Polymerase Chain Reaction; Single cell oil; Strain; Sugars; Yeast; yeasts
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2012.08.024

[Display omitted] ► The inulinase-producing yeast strain Pcla22 of Pichia guilliermondii was obtained. ► Lipid content in the yeast cells cultivated a flask reached 55.2%. ► 60.6% of lipid was obtained after the fed-batch fermentation. ► Many lipid bodies were produced in the yeast cells. In this study, an inulinase-producing yeast strain Pcla22 of Pichia guilliermondii was identified. It was found that the yeast strain Pcla22 could produce higher amount of oil and more lipid bodies in its cells than any other yeast strains tested in this study. Under the optimal conditions, 60.6%(w/w) of lipid based on cell dry weight, 20.4g/l of the dry cell mass, SCO produced per g of consumed sugar of 0.19g/g and biomass produced per g of consumed sugar of 0.32g/g were obtained in the culture of the yeast strain Pcla22 after 96h of the fed-batch fermentation. Over 79.8% of the fatty acids from the yeast strain Pcla22 grown in the oil production medium containing inulin was C16:0 and C18:1, especially C18:1 (57.9%). The biodiesel obtained from the produced lipid could be burnt well.