Efficient saccharification for non-treated cassava pulp by supplementation of Clostridium thermocellum cellulosome and Thermoanaerobacter brockii β-glucosidase

Cassava pulp containing 60% starch and 20% cellulose is a promising renewable source for bioethanol. The starch granule was observed to tightly bind cellulose fiber. To achieve an efficient degradation for cassava pulp, saccharification tests without pre-gelatinization treatment were carried out usi...

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Bibliographic Details
Published in:Bioresource technology 2013-03, Vol.132, p.383-386
Main Authors: Vaithanomsat, Pilanee, Kosugi, Akihiko, Apiwatanapiwat, Waraporn, Thanapase, Warunee, Waeonukul, Rattiya, Tachaapaikoon, Chakrit, Pason, Patthra, Mori, Yutaka
Format: Article
Language:English
Subjects:
alpha-Amylases - metabolism
Amylolytic enzymes
beta-Glucosidase - metabolism
Biofuels
Biological and medical sciences
Biotechnology - methods
Carbohydrates - biosynthesis
Cassava
cellulose
Cellulose fibers
cellulosic fibers
Cellulosome
Cellulosomes - enzymology
Cellulosomes - genetics
Chromatography, Gas
Chromatography, High Pressure Liquid
Clostridium thermocellum
Clostridium thermocellum - enzymology
DNA Primers - genetics
Drying
Fundamental and applied biological sciences. Psychology
glucan 1,4-alpha-glucosidase
Glucan 1,4-alpha-Glucosidase - genetics
Glucan 1,4-alpha-Glucosidase - metabolism
Granular materials
Granules
Manihot - chemistry
Manihot - metabolism
Non-treated cassava pulp
pulp
Recombinant Proteins - genetics
Saccharification
starch granules
Starches
Thermoanaerobacter - enzymology
Thermoanaerobacter brockii
Thermophilic β-glucosidase
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Summary:Cassava pulp containing 60% starch and 20% cellulose is a promising renewable source for bioethanol. The starch granule was observed to tightly bind cellulose fiber. To achieve an efficient degradation for cassava pulp, saccharification tests without pre-gelatinization treatment were carried out using combination of commercial α-amylase with cellulosome from Clostridium thermocellum S14 and β-glucosidase (rCglT) from Thermoanaerobacter brockii. The saccharification rate for cassava pulp was shown 59% of dry matter. To obtain maximum saccharification rate, glucoamylase (GA) from C. thermocellum S14 was supplemented to the combination. The result showed gradual increase in the saccharification rate to 74% (dry matter). Supplementation of GA to the combination of commercial α-amylase, cellulosome and rCglT is powerful method for efficient saccharification of cassava pulp without pretreatment.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2012.11.023