Development of InDel markers linked to Fusarium wilt resistance in cabbage

Cabbage Fusarium wilt (CFW) is a destructive disease causing great losses to cabbage (Brassica oleracea L. var. capitata L.) production worldwide. At present, there are few reports concerning molecular marker research on cabbage resistance to CFW. In this study, 160 double haploid (DH) lines were ob...

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Bibliographic Details
Published in:Molecular breeding 2013-12, Vol.32 (4), p.961-967
Main Authors: Lv, Hong-hao, Yang, Li-mei, Kang, Jun-gen, Wang, Qing-biao, Wang, Xiao-wu, Fang, Zhi-yuan, Liu, Yu-mei, Zhuang, Mu, Zhang, Yang-yong, Lin, Yan, Yang, Yu-hong, Xie, Bing-yan, Liu, Bo, Liu, Ji-Sheng
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biotechnology
Brassica
Brassica oleracea
cabbage
chromosome mapping
Chromosomes
Clonal deletion
Cloning
Fusarium
Fusarium oxysporum
Fusarium wilt
Gene mapping
Gene sequencing
genes
Genomes
haploidy
inbred lines
Inbreeding
Insertion
Life Sciences
Mapping
Marker-assisted selection
Markers
Molecular biology
Nucleotide sequence
nucleotide sequences
parents
phenotype
Phenotypes
Plant biology
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Plant Sciences
Wilt
wilting
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Summary:Cabbage Fusarium wilt (CFW) is a destructive disease causing great losses to cabbage (Brassica oleracea L. var. capitata L.) production worldwide. At present, there are few reports concerning molecular marker research on cabbage resistance to CFW. In this study, 160 double haploid (DH) lines were obtained from the F1 population of a 99–77 (highly resistant to CFW) × 99–91 (highly susceptible to CFW) cross. Insertion–deletion (InDel) markers were designed according to the reference genome sequence of cabbage and the whole-genome re-sequencing data of the two parents. A genetic map of chromosome C06 including seven InDel markers was constructed based on the DH population. Thus, FOC (resistance gene to Fusarium oxysporum f. sp. conglutinans) was located on chromosome C06 and two InDel markers out of the seven, M10 and A1, flanked the gene at 1.2 and 0.6 cM, respectively. Marker A1 revealed a significant consistency with the phenotype assay in the F2 population as well as in 40 inbred lines (96 and 82 %, respectively). This study lays the foundation for fine mapping and cloning of the FOC gene and for marker-assisted selection in cabbage resistance breeding.
ISSN:1380-3743
1572-9788
DOI:10.1007/s11032-013-9925-x