Combinatorial Approach for Fabrication of Coatings to Control Bacterial Adhesion

Due to the high importance of bacterial infections in medical devices there is an increasing interest in the design of anti-fouling coatings. The application of substrates with controlled chemical gradients to prevent microbial adhesion is presented. We describe here the co-polymerization of poly(et...

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Bibliographic Details
Published in:Journal of biomaterials science. Polymer ed. 2012-01, Vol.23 (12), p.1613-1628
Main Authors: Pedron, S., Peinado, C., Catalina, F., Bosch, P., Anseth, K.S., Abrusci, C.
Format: Article
Language:English
Subjects:
Adhesion
Anti-fouling
Bacteria
bacterial adhesion' surface gradients
Biomedical materials
Coatings
Combinatorial analysis
Copolymerization
cross-linking gradient
Escherichia coli
hyperbranched polymers
Medical devices
microfluidics
Microorganisms
Pseudomonas aeruginosa
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Summary:Due to the high importance of bacterial infections in medical devices there is an increasing interest in the design of anti-fouling coatings. The application of substrates with controlled chemical gradients to prevent microbial adhesion is presented. We describe here the co-polymerization of poly(ethylene glycol) dimethacrylate with a hyperbranched multimethacrylate (H30MA) using a chemical gradient generator; and the resulting films were characterized with respect to their ability to serve as coating for biomedical devices. The photo-polymerized materials present special surface properties due to the hyperbranched structure of H30MA and phase separation at specific concentrations in the PEGDM matrix. This approach affords the investigation of cell response to a large range of different chemistries on a single sample. Two bacterial strains commonly associated with surgical site infections, Escherichia coli and Pseudomonas aeruginosa, have been cultured on these substrates to study their attachment behaviour. These gradient-coated samples demonstrate less bacterial adhesion at higher concentrations of H30MA, and the adhesion is substantially affected by the extent of surface phase segregation.
ISSN:0920-5063
1568-5624
DOI:10.1163/092050611X589329