Maternal immune activation affects litter success, size and neuroendocrine responses related to behavior in adult offspring

• Published in: Physiology & behavior 2013-07, Vol.119, p.175-184
• Main Authors: French, Susannah S, Chester, Emily M, Demas, Gregory E
• Format: Article
• Language: English
• Subjects: adulthood; adults; Animals; Behavioral psychophysiology; Biological and medical sciences; correlation; Cortisol; Cricetinae; defensive behavior; Dose-Response Relationship, Drug; Epigenetic effects; Female; females; Fertility - drug effects; Fertility - immunology; Fundamental and applied biological sciences. Psychology; hamsters; Hydrocortisone - blood; immune response; immunocompetence; Immunoglobulin G - blood; Lipopolysaccharide (LPS); lipopolysaccharides; Lipopolysaccharides - administration & dosage; Lipopolysaccharides - immunology; Lipopolysaccharides - pharmacology; litter size; Litter Size - drug effects; Litter Size - immunology; Male; maternal effect; microorganisms; pathogens; phenotype; Pregnancy; Prenatal Exposure Delayed Effects - blood; Prenatal Exposure Delayed Effects - chemically induced; Prenatal Exposure Delayed Effects - immunology; progeny; Psychiatry; Psychology. Psychoanalysis. Psychiatry; Psychology. Psychophysiology; Social Behavior; Testosterone; Testosterone - blood
• ISSN: 0031-9384; 1873-507X
• DOI: 10.1016/j.physbeh.2013.06.018

Abstract It is increasingly evident that influences other than genetics can contribute to offspring phenotype. In particular, maternal influences are an important contributing factor to offspring survival, development, physiology and behavior. Common environmental pathogens such as viral or bacterial microorganisms can induce maternal immune responses, which have the potential to alter the prenatal environment via multiple independent pathways. The effects of maternal immune activation on endocrine responses and behavior are less well studied and provide the basis for the current study. Our approach in the current study was two-pronged: 1) quantify sickness responses during pregnancy in adult female hamsters experiencing varying severity of immune responsiveness (i.e., differing doses of lipopolysaccharide [LPS]), and 2) assess the effects of maternal immune activation on offspring development, immunocompetence, hormone profiles, and social behavior during adulthood. Pregnancy success decreased with increasing doses of LPS, and litter size was reduced in LPS dams that managed to successfully reproduce. Unexpectedly, pregnant females treated with LPS showed a hypothermic response in addition to the more typical anorexic and body mass changes associated with sickness. Significant endocrine changes related to behavior were observed in the offspring of LPS-treated dams; these effects were apparent in adulthood. Specifically, offspring from LPS treated dams showed significantly greater cortisol responses to stressful resident–intruder encounters compared with offspring from control dams. Post-behavior cortisol was elevated in male LPS offspring relative to the offspring of control dams, and was positively correlated with the frequency of bites during agonistic interactions, and cortisol levels in both sexes were related to defensive behaviors, suggesting that changes in hypothalamo–pituitary–adrenal axis responsiveness may play a regulatory role in the observed behavioral differences. Overall, the results of this study provide evidence that maternal immune activation can exert marked effects on offspring physiology and behavior.