Bacterial adhesion on the titanium and zirconia abutment surfaces

• Published in: Clinical oral implants research 2014-03, Vol.25 (3), p.337-343
• Main Authors: Nascimento, Cássio do, Pita, Murillo Sucena, Fernandes, Flávio Henrique Nogueira Carriço, Pedrazzi, Vinícius, de Albuquerque Junior, Rubens Ferreira, Ribeiro, Ricardo Faria
• Format: Article
• Language: English
• Subjects: Adhesion; Adult; Bacteria; Bacterial Adhesion; Biofilms; biomaterials; clinical assessment; clinical research; Colonization; Colony Count, Microbial; Cross-Over Studies; Dental Abutments; Dentistry; Deoxyribonucleic acid; diagnosis; Disks; DNA; Etiology; Forsythia; Fusobacterium; Humans; Hybridization; In vivo methods and tests; Materials Testing; microbiology; Microorganisms; Mucosa; Oral cavity; Pathogens; Periodontal diseases; prosthodontics; Species; Streptococcus gordonii; Streptococcus parasanguinis; Substrates; Surface Properties; Surgical implants; Titanium; Titanium - chemistry; Zirconia; Zirconium - chemistry; Zirconium dioxide
• ISSN: 0905-7161; 1600-0501
• DOI: 10.1111/clr.12093

Objective Microorganisms harboring the oral cavity, mainly those related to periodontal diseases, are the most potential etiologic factor of failure in long‐term implant treatment. The material used for abutment components may influence the adhesion and colonization of microbial species. The aim of this in vivo investigation was to evaluate the biofilm formation on machined (MPT) or cast titanium (CPT) and zirconia abutments (Zc). Methods Six healthy subjects were enrolled in this randomized crossover clinical investigation. The study was conducted in three phases according to abutment surface evaluated. Each subject used an individual oral splint containing four disks of the same tested substrate, two located in the anterior and two in the posterior region, totalizing 12 specimens for subject. Participants were asked to use the removable intraoral splint during 24 h. DNA checkerboard hybridization method was used to identify and quantify 38 bacterial species colonizing formed biofilm on the abutment substrates. Results Pathogens and non‐pathogens species were found colonizing the three substrates surfaces. Fusobacterium nucleatum, Neisseria mucosa, Porphyromonas aeruginosa, Peptostreptococcus anaerobios, Staphylococcus aureus, Streptococcus gordonii, Streptococcus parasanguinis, and Tanerella forsythia were the only species with no significant differences over the tested materials (P > 0.05). All the other target species presented significant differences sought by Friedman test (P