Determination of pharmacokinetics of cocaine in sheep by liquid chromatography

Cocaine was administered as 1-, 2-, and 4-mg/kg intravenous bolus doses to each of six sheep. Plasma samples were collected as a function of time and assayed for cocaine using reversed-phase ion-pair HPLC. The assay involved double extraction with ether and UV detection at 229 nm. Using 2 mL of plas...

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Bibliographic Details
Published in:Journal of pharmaceutical sciences 1988-01, Vol.76 (1), p.39-43
Main Authors: Khan, M, Gupta, P K, Cristie, R, Nangia, A, Winter, H, Lam, F C, Perrier, D G, Hung, C T
Format: Article
Language:English
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Summary:Cocaine was administered as 1-, 2-, and 4-mg/kg intravenous bolus doses to each of six sheep. Plasma samples were collected as a function of time and assayed for cocaine using reversed-phase ion-pair HPLC. The assay involved double extraction with ether and UV detection at 229 nm. Using 2 mL of plasma, levels of 1 ng/ml of cocaine can be measured. The concentration versus time data obtained for the plasma samples were analyzed by a "noncompartmental" method. The pharmacokinetic parameters of cocaine in sheep had mean values of 4.0 L/kg, 3.5 L/kd, and 0.29 L multiplied by min super(-1) multiplied by kg super(-1) for Vd, Vd sub(ss), and CL respectively. The clearance of cocaine in sheep was much higher than cardiac output. Pulmonary first-pass effect has been suggested as the possible explanation for the large clearance of cocaine in sheep.
ISSN:0022-3549