Arginine methylation of CRTC2 is critical in the transcriptional control of hepatic glucose metabolism

Fasting glucose homeostasis is maintained in part through cAMP (adenosine 3',5'-monophosphate)-dependent transcriptional control of hepatic gluconeogenesis by the transcription factor CREB (cAMP response element-binding protein) and its coactivator CRTC2 (CREB-regulated transcriptional coa...

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Published in:Science signaling 2014-02, Vol.7 (314), p.ra19-ra19
Main Authors: Han, Hye-Sook, Jung, Chang-Yun, Yoon, Young-Sil, Choi, Seri, Choi, Dahee, Kang, Geon, Park, Keun-Gyu, Kim, Seong-Tae, Koo, Seung-Hoi
Format: Article
Language:English
Subjects:
Animals
Arginine - genetics
Arginine - metabolism
Cell Line
Cyclic AMP - genetics
Cyclic AMP - metabolism
Gluconeogenesis
Glucose - genetics
Glucose - metabolism
Humans
Insulin Resistance
Liver - metabolism
Liver - pathology
Methylation
Mice
Obesity - genetics
Obesity - metabolism
Obesity - pathology
Protein-Arginine N-Methyltransferases - genetics
Protein-Arginine N-Methyltransferases - metabolism
Transcription Factors - genetics
Transcription Factors - metabolism
Transcription, Genetic
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Summary:Fasting glucose homeostasis is maintained in part through cAMP (adenosine 3',5'-monophosphate)-dependent transcriptional control of hepatic gluconeogenesis by the transcription factor CREB (cAMP response element-binding protein) and its coactivator CRTC2 (CREB-regulated transcriptional coactivator 2). We showed that PRMT6 (protein arginine methyltransferase 6) promotes fasting-induced transcriptional activation of the gluconeogenic program involving CRTC2. Mass spectrometric analysis indicated that PRMT6 associated with CRTC2. In cells, PRMT6 mediated asymmetric dimethylation of multiple arginine residues of CRTC2, which enhanced the association of CRTC2 with CREB on the promoters of gluconeogenic enzyme-encoding genes. In mice, ectopic expression of PRMT6 promoted higher blood glucose concentrations, which were associated with increased expression of genes encoding gluconeogenic factors, whereas knockdown of hepatic PRMT6 decreased fasting glycemia and improved pyruvate tolerance. The abundance of hepatic PRMT6 was increased in mouse models of obesity and insulin resistance, and adenovirus-mediated depletion of PRMT6 restored euglycemia in these mice. We propose that PRMT6 is involved in the regulation of hepatic glucose metabolism in a CRTC2-dependent manner.
ISSN:1945-0877
1937-9145
DOI:10.1126/scisignal.2004479