Antibody humanization methods - a review and update

This article reviews recent advances achieved during recent years on various aspects of antibody humanization theories and techniques. Common methods for producing humanized antibodies including framework-homology-based humanization, germline humanization, complementary determining regions (CDR)-hom...

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Bibliographic Details
Published in:Biotechnology & genetic engineering reviews 2013-01, Vol.29 (2), p.175-186
Main Authors: Safdari, Yaghoub, Farajnia, Safar, Asgharzadeh, Mohammad, Khalili, Masoumeh
Format: Article
Language:English
Subjects:
affinity
Amino Acid Sequence
Antibodies, Monoclonal, Humanized - chemistry
Antibodies, Monoclonal, Humanized - classification
Antibodies, Monoclonal, Humanized - genetics
Antibodies, Monoclonal, Humanized - immunology
Antibodies, Monoclonal, Humanized - therapeutic use
CDR homology
Complementarity Determining Regions - immunology
framework homology
Germ Cells - immunology
germline humanization
humanized antibodies
Humans
immunogenicity
Protein Engineering - methods
SDR grafting
Sensitivity and Specificity
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Summary:This article reviews recent advances achieved during recent years on various aspects of antibody humanization theories and techniques. Common methods for producing humanized antibodies including framework-homology-based humanization, germline humanization, complementary determining regions (CDR)-homology-based humanization and specificity determining residues (SDR) grafting, as well as advantages and disadvantages of each of these methods and their applications are discussed.
ISSN:0264-8725
2046-5556
DOI:10.1080/02648725.2013.801235