Base pair mismatches and carcinogen-modified bases in DNA: An NMR study of G multiplied by T and G multiplied by O super(4)meT pairing in dodecanucleotide duplexes

High-resolution two-dimensional NMR studies have been completed on the self-complementary d(C-G-C-G-A-G-C-T-T-G-C-G) duplex (designated G multiplied by T 12-mer) and the self-complementary d(C-G-C-G-A-G-C-T-O super(4)meT-G-C-G) duplex (designated G multiplied by O super(4)meT 12-mer) containing G mu...

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Bibliographic Details
Published in:Biochemistry (Easton) 1988-01, Vol.27 (1), p.108-115
Main Authors: Kalnik, M W, Kouchakdjian, M, Li, BFL, Swann, P F, Patel, D J
Format: Article
Language:English
Online Access:Get full text
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Summary:High-resolution two-dimensional NMR studies have been completed on the self-complementary d(C-G-C-G-A-G-C-T-T-G-C-G) duplex (designated G multiplied by T 12-mer) and the self-complementary d(C-G-C-G-A-G-C-T-O super(4)meT-G-C-G) duplex (designated G multiplied by O super(4)meT 12-mer) containing G multiplied by T and G multiplied by O super(4)meT pairs at identical positions four base pairs in from either end of the duplex. The exchangeable and nonexchangeable proton resonances have been assigned from an analysis of two-dimensional nuclear Overhauser enhancement (NOESY) spectra for the G multiplied by T 12-mer and G multiplied by O super(4)meT 12-mer duplexes in H sub(2)O and D sub(2)O solution. The authors propose an alternate base pairing mode stabilized by one short hydrogen bond between the 2-amino group of guanosine and the 2-carbonyl group of O super(4)meT.
ISSN:0006-2960