Tissue expression pattern and polymorphism of G0S2 gene in porcine

• Published in: Gene 2014-04, Vol.539 (1), p.173-179
• Main Authors: Jiang, Yanzhi, Cen, Wangmin, Xing, Shuhua, Chen, Jianning, Xu, Huaming, Wen, Anxiang, Zhu, Li, Tang, Guoqing, Li, Mingzhou, Jiang, Anan, Li, Xuewei
• Format: Article
• Language: English
• Subjects: Adipocytes - enzymology; Adipocytes - metabolism; Adipose Tissue - enzymology; Adipose triglyceride lipase (ATGL); Animals; Association analysis; Base Sequence; Body Composition - genetics; Cell Cycle Proteins - genetics; Female; G0/G1 switch gene 2 (G0S2); Genetic Variation; Lipase - genetics; Liver - enzymology; Male; Polymorphism; Polymorphism, Single Nucleotide; Porcine; RNA, Messenger - metabolism; Sequence Analysis, DNA; Subcutaneous Fat - metabolism; Swine - genetics; Tissue Distribution; Tissue expression; Transcription, Genetic
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/j.gene.2014.01.058

Adipose triglyceride lipase (ATGL), catalyzing the initial step of hydrolysis of triacylglycerol (TAG) in adipocytes, has been known to be inhibited by G0/G1 switch protein 2 (G0S2). In this study, we determined tissue expression pattern and polymorphism of G0S2 gene in porcine. The results showed that the G0S2 transcript levels were very high in the liver and, to a lesser degree, in adipose tissues of greater omentum and suet fat; and low G0S2 transcript levels were observed in other tissues. A comparative study on the transcript levels between ATGL and G0S2 genes showed that ATGL transcript levels were high in all six adipose tissues, but negligible in the liver. Higher transcript levels were obtained for sows in adipose tissues of the inner layer of subcutaneous fat and suet fat, but higher expression values were found for boars in the liver, spleen, and stomach. 19 single nucleotide polymorphisms (SNPs), including 4 nonsynonymous SNPs (g.-307A>T, g.-394C>G, g.-565G>A, and g.-566T>C), were found in porcine G0S2 genomic DNA. Association analyses showed that the g.-565G>A and g.-742T>A SNPs were associated with back fat thickness (BFT). In conclusion, G0S2 mRNAs are abundantly expressed in porcine liver and adipose tissues of greater omentum and suet fat, and sex affects porcine G0S2 tissue transcript levels; meanwhile, the genetic diversity of porcine G0S2 gene is abundant and 2 SNPs are a genetic factor affecting BFT. •The mRNA levels of G0S2 are high in porcine liver and two adipose tissues.•The expression pattern of porcine G0S2 and ATGL are different in the liver.•Sex affects porcine G0S2 transcript levels. 19 SNPs were found in porcine G0S2 genomic DNA.•The g.-565G>A and g.-742T>A SNPs are associated with BFT.