Whole-cell fluorescence for observing reductive metabolism from carbon compounds and hydrogen in Azotobacter vinelandii

The change in fluorescence of intact Azotobacter vinelandii was observed to study the oxidation and reduction of flavin and pyridine nucleotides resulting from carbon and hydrogen metabolism. Metronidazole, acetaldehyde, and oxygen each oxidized flavin. Flavin oxidized by metronidazole or acetaldehy...

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Bibliographic Details
Published in:Current microbiology 1988-01, Vol.17 (4), p.209-214
Main Authors: Peterson, J.B, LaRue, T.A
Format: Article
Language:English
Subjects:
AZOTOBACTER
Azotobacter vinelandii
BACTERIA
BACTERIE
CARBON
CARBONE
CARBONO
FIJACION DEL NITROGENO
FIXATION DE L'AZOTE
HIDROGENO
HYDROGEN
HYDROGENE
NITROGEN FIXATION
OPTICAL PROPERTIES
PROPIEDADES OPTICAS
PROPRIETE OPTIQUE
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Summary:The change in fluorescence of intact Azotobacter vinelandii was observed to study the oxidation and reduction of flavin and pyridine nucleotides resulting from carbon and hydrogen metabolism. Metronidazole, acetaldehyde, and oxygen each oxidized flavin. Flavin oxidized by metronidazole or acetaldehyde was reduced by addition of mannitol or ethanol, but not by acetate or hydrogen. The fluorescence induced by oxygen was transient. Mannitol, ethanol, acetate, acetaldehyde, and hydrogen shortened the duration of the oxygen-dependent fluorescence and supported respiration. The changes in redox state of pyridine nucleotides corresponded to the changes in flavin redox state. This indicates that the use of reducing equivalents from uptake hydrogenase is limited to the respiratory electron transport system in Azotobacter vinelandii .
ISSN:0343-8651
1432-0991
DOI:10.1007/BF01589454