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Whole-cell fluorescence for observing reductive metabolism from carbon compounds and hydrogen in Azotobacter vinelandii
The change in fluorescence of intact Azotobacter vinelandii was observed to study the oxidation and reduction of flavin and pyridine nucleotides resulting from carbon and hydrogen metabolism. Metronidazole, acetaldehyde, and oxygen each oxidized flavin. Flavin oxidized by metronidazole or acetaldehy...
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Published in: | Current microbiology 1988-01, Vol.17 (4), p.209-214 |
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container_title | Current microbiology |
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creator | Peterson, J.B LaRue, T.A |
description | The change in fluorescence of intact Azotobacter vinelandii was observed to study the oxidation and reduction of flavin and pyridine nucleotides resulting from carbon and hydrogen metabolism. Metronidazole, acetaldehyde, and oxygen each oxidized flavin. Flavin oxidized by metronidazole or acetaldehyde was reduced by addition of mannitol or ethanol, but not by acetate or hydrogen. The fluorescence induced by oxygen was transient. Mannitol, ethanol, acetate, acetaldehyde, and hydrogen shortened the duration of the oxygen-dependent fluorescence and supported respiration. The changes in redox state of pyridine nucleotides corresponded to the changes in flavin redox state. This indicates that the use of reducing equivalents from uptake hydrogenase is limited to the respiratory electron transport system in Azotobacter vinelandii . |
doi_str_mv | 10.1007/BF01589454 |
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Metronidazole, acetaldehyde, and oxygen each oxidized flavin. Flavin oxidized by metronidazole or acetaldehyde was reduced by addition of mannitol or ethanol, but not by acetate or hydrogen. The fluorescence induced by oxygen was transient. Mannitol, ethanol, acetate, acetaldehyde, and hydrogen shortened the duration of the oxygen-dependent fluorescence and supported respiration. The changes in redox state of pyridine nucleotides corresponded to the changes in flavin redox state. This indicates that the use of reducing equivalents from uptake hydrogenase is limited to the respiratory electron transport system in Azotobacter vinelandii .</description><identifier>ISSN: 0343-8651</identifier><identifier>EISSN: 1432-0991</identifier><identifier>DOI: 10.1007/BF01589454</identifier><language>eng</language><subject>AZOTOBACTER ; Azotobacter vinelandii ; BACTERIA ; BACTERIE ; CARBON ; CARBONE ; CARBONO ; FIJACION DEL NITROGENO ; FIXATION DE L'AZOTE ; HIDROGENO ; HYDROGEN ; HYDROGENE ; NITROGEN FIXATION ; OPTICAL PROPERTIES ; PROPIEDADES OPTICAS ; PROPRIETE OPTIQUE</subject><ispartof>Current microbiology, 1988-01, Vol.17 (4), p.209-214</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Peterson, J.B</creatorcontrib><creatorcontrib>LaRue, T.A</creatorcontrib><title>Whole-cell fluorescence for observing reductive metabolism from carbon compounds and hydrogen in Azotobacter vinelandii</title><title>Current microbiology</title><description>The change in fluorescence of intact Azotobacter vinelandii was observed to study the oxidation and reduction of flavin and pyridine nucleotides resulting from carbon and hydrogen metabolism. Metronidazole, acetaldehyde, and oxygen each oxidized flavin. Flavin oxidized by metronidazole or acetaldehyde was reduced by addition of mannitol or ethanol, but not by acetate or hydrogen. The fluorescence induced by oxygen was transient. Mannitol, ethanol, acetate, acetaldehyde, and hydrogen shortened the duration of the oxygen-dependent fluorescence and supported respiration. The changes in redox state of pyridine nucleotides corresponded to the changes in flavin redox state. This indicates that the use of reducing equivalents from uptake hydrogenase is limited to the respiratory electron transport system in Azotobacter vinelandii .</description><subject>AZOTOBACTER</subject><subject>Azotobacter vinelandii</subject><subject>BACTERIA</subject><subject>BACTERIE</subject><subject>CARBON</subject><subject>CARBONE</subject><subject>CARBONO</subject><subject>FIJACION DEL NITROGENO</subject><subject>FIXATION DE L'AZOTE</subject><subject>HIDROGENO</subject><subject>HYDROGEN</subject><subject>HYDROGENE</subject><subject>NITROGEN FIXATION</subject><subject>OPTICAL PROPERTIES</subject><subject>PROPIEDADES OPTICAS</subject><subject>PROPRIETE OPTIQUE</subject><issn>0343-8651</issn><issn>1432-0991</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><recordid>eNotz71LAzEcxvEgCtbq4uiUye00r5fcWItVoeCgxbHkkl_ayF2iyV1F_3ordXqWD194ELqk5IYSom7vFoRK3QgpjtCECs4q0jT0GE0IF7zStaSn6KyUd0IoawidoK-3beqgstB12HdjylAsRAvYp4xTWyDvQtzgDG60Q9gB7mEwbepC6bHPqcfW5DZFbFP_kcboCjbR4e23y2kDEYeIZz9pSK2xA2S8b0G3ByGcoxNvugIX_ztFq8X96_yxWj4_PM1ny8ozwoeqqaXTznnuNW-toVwb6pSm1illlRRCMEaVZC1ziknhmXC1t9R7WosGmOJTdH3ofuT0OUIZ1n0of29NhDSWNZWE17rRe3h1gN6ktdnkUNarF61rpmTDfwEr2mkH</recordid><startdate>19880101</startdate><enddate>19880101</enddate><creator>Peterson, J.B</creator><creator>LaRue, T.A</creator><scope>FBQ</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>19880101</creationdate><title>Whole-cell fluorescence for observing reductive metabolism from carbon compounds and hydrogen in Azotobacter vinelandii</title><author>Peterson, J.B ; LaRue, T.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f203t-965d8ddf3f83bca138a1d781cd77c75444221752b2d7254f24d6fc1ff1649e273</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>AZOTOBACTER</topic><topic>Azotobacter vinelandii</topic><topic>BACTERIA</topic><topic>BACTERIE</topic><topic>CARBON</topic><topic>CARBONE</topic><topic>CARBONO</topic><topic>FIJACION DEL NITROGENO</topic><topic>FIXATION DE L'AZOTE</topic><topic>HIDROGENO</topic><topic>HYDROGEN</topic><topic>HYDROGENE</topic><topic>NITROGEN FIXATION</topic><topic>OPTICAL PROPERTIES</topic><topic>PROPIEDADES OPTICAS</topic><topic>PROPRIETE OPTIQUE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Peterson, J.B</creatorcontrib><creatorcontrib>LaRue, T.A</creatorcontrib><collection>AGRIS</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Current microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Peterson, J.B</au><au>LaRue, T.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Whole-cell fluorescence for observing reductive metabolism from carbon compounds and hydrogen in Azotobacter vinelandii</atitle><jtitle>Current microbiology</jtitle><date>1988-01-01</date><risdate>1988</risdate><volume>17</volume><issue>4</issue><spage>209</spage><epage>214</epage><pages>209-214</pages><issn>0343-8651</issn><eissn>1432-0991</eissn><abstract>The change in fluorescence of intact Azotobacter vinelandii was observed to study the oxidation and reduction of flavin and pyridine nucleotides resulting from carbon and hydrogen metabolism. Metronidazole, acetaldehyde, and oxygen each oxidized flavin. Flavin oxidized by metronidazole or acetaldehyde was reduced by addition of mannitol or ethanol, but not by acetate or hydrogen. The fluorescence induced by oxygen was transient. Mannitol, ethanol, acetate, acetaldehyde, and hydrogen shortened the duration of the oxygen-dependent fluorescence and supported respiration. The changes in redox state of pyridine nucleotides corresponded to the changes in flavin redox state. This indicates that the use of reducing equivalents from uptake hydrogenase is limited to the respiratory electron transport system in Azotobacter vinelandii .</abstract><doi>10.1007/BF01589454</doi><tpages>6</tpages></addata></record> |
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subjects | AZOTOBACTER Azotobacter vinelandii BACTERIA BACTERIE CARBON CARBONE CARBONO FIJACION DEL NITROGENO FIXATION DE L'AZOTE HIDROGENO HYDROGEN HYDROGENE NITROGEN FIXATION OPTICAL PROPERTIES PROPIEDADES OPTICAS PROPRIETE OPTIQUE |
title | Whole-cell fluorescence for observing reductive metabolism from carbon compounds and hydrogen in Azotobacter vinelandii |
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