Tracking of Single Receptor Molecule Mobility in Neuronal Membranes: A Quick Theoretical and Practical Guide

Single‐molecule detection enables us to visualise the real‐time dynamics of individual molecules in live cells. We review the recent advancements in single‐molecule fluorescence tracking of receptor protein mobility in the neuronal membrane. First, we discuss the practical consideration of single‐mo...

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Bibliographic Details
Published in:Journal of neuroendocrinology 2013-11, Vol.25 (11), p.1231-1237
Main Authors: Kwakowsky, A., Potapov, D., Ábrahám, I. M.
Format: Article
Language:English
Subjects:
Animals
Cell Membrane - ultrastructure
fluorophores
Membrane Proteins - ultrastructure
Molecular Imaging - instrumentation
Molecular Imaging - methods
neurones
Neurons - ultrastructure
receptors
single-molecule tracking
total internal reflection fluorescence microscopy
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Summary:Single‐molecule detection enables us to visualise the real‐time dynamics of individual molecules in live cells. We review the recent advancements in single‐molecule fluorescence tracking of receptor protein mobility in the neuronal membrane. First, we discuss the practical consideration of single‐molecule tracking in neurones, including the choice of cells and possible fluorescent labelling, as well as the appropriate optical set‐up and imaging technology. We then describe the analysis of the single‐molecule imaging data, including its theoretical and practical aspects of and relevant estimations of the biophysical parameters. Finally, we provide an example of a single‐molecule tracking study in neuroendocrinology and highlight the next frontiers of single‐molecule detection technologies.
ISSN:0953-8194
1365-2826
DOI:10.1111/jne.12083