Disposable amperometric biosensor based on lactate oxidase immobilised on platinum nanoparticle-decorated carbon nanofiber and poly(diallyldimethylammonium chloride) films

• Published in: Biosensors & bioelectronics 2014-06, Vol.56, p.345-351
• Main Authors: Lamas-Ardisana, Pedro J., Loaiza, Oscar A., Añorga, Larraitz, Jubete, Elena, Borghei, Maryam, Ruiz, Virginia, Ochoteco, Estibalitz, Cabañero, Germán, Grande, Hans J.
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Biosensing Techniques - methods; Biosensors; Biotechnology; Carbon - chemistry; Carbon nanofiber; Enzymes, Immobilized - chemistry; Enzymes, Immobilized - metabolism; Fundamental and applied biological sciences. Psychology; Humans; Lactate; Lactate oxidase; Lactic Acid - analysis; Lactic Acid - blood; Lactic Acid - metabolism; Limit of Detection; Methods. Procedures. Technologies; Mixed Function Oxygenases - chemistry; Mixed Function Oxygenases - metabolism; Nanofibers - chemistry; Nanofibers - ultrastructure; Pediococcus - enzymology; Platinum - chemistry; Platinum nanoparticle; Polyethylenes - chemistry; Quaternary Ammonium Compounds - chemistry; Reproducibility of Results; Screen-printed electrode; Sweat; Sweat - chemistry; Various methods and equipments
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2014.01.047

A novel biosensor for lactate has been developed, using screen-printed carbon electrodes (SPCE) and lactate oxidase (LOx). The active surface of the electrodes was modified using a dispersion of platinum nanoparticle decorated carbon nanofibers (PtNp-CNF) in poly(diallyldimethylammonium) chloride (PDDA) solution. In this way, sensitive, disposable, low cost and reliable hydrogen peroxide sensors were obtained. The immobilisation of LOx on top of these PtNp-CNF-PDDA/SPCEs resulted in amperometric biosensors with high operational stability. The sensitivity of the optimised lactate biosensor was 36.8 (mA/Mcm2) with a linear range of 25–1500µM. The limit of detection was 11µM (S/N=3). Reproducibility, selectivity and storage stability were also evaluated. Additionally, the stability of the biosensor was also predicted by a model based on thermal degradation. Finally, lactate in sweat and blood samples was determined in a sport test using LOx/PtNp-CNF-PDDA/SPCEs and commercial biosensors respectively. Based on these data, the validity of the sweat lactate for the determination of the lactate threshold is discussed. •Determination of lactate based on hydrogen peroxide amperometric detection is carried out by screen-printed carbon electrodes (SPCE) modified with a film of platinum nanoparticle decorated carbon nanofibers (PtNp-CNF) in poly(diallyldimethylammonium) chloride (PDDA).•The performance of the biosensor in real sweat samples is evaluated.•The long term stability of the biosensors is predicted by a model based on thermal degradation.•The validity of the sweat lactate for the determination of the lactate threshold is discussed.