A decadentate Gd(III)-coordinating paramagnetic cosolvent for protein relaxation enhancement measurement

Solvent paramagnetic relaxation enhancement (sPRE) arises from random collisions between paramagnetic cosolvent and protein of interest. The sPRE can be readily measured, affording protein structure information. However, lack of an inert cosolvent probe may yield sPRE values that are not consistent...

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Published in:Journal of biomolecular NMR 2014-03, Vol.58 (3), p.149-154
Main Authors: Gu, Xin-Hua, Gong, Zhou, Guo, Da-Chuan, Zhang, Wei-Ping, Tang, Chun
Format: Article
Language:English
Subjects:
Biochemistry
Biological and Medical Physics
Biophysics
Communication
Edetic Acid - analogs & derivatives
Edetic Acid - chemistry
Electron Spin Resonance Spectroscopy - methods
Gadolinium
Gadolinium - chemistry
Magnetic Resonance Spectroscopy
Nuclear Magnetic Resonance, Biomolecular - methods
Physics
Physics and Astronomy
Proteins - chemistry
Proteins - ultrastructure
Spectroscopy/Spectrometry
Water - chemistry
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cites cdi_FETCH-LOGICAL-c372t-d094055a336104d3c0a3700abcae758a0fed19286ea570c9bae9095b354698303
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container_title Journal of biomolecular NMR
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creator Gu, Xin-Hua
Gong, Zhou
Guo, Da-Chuan
Zhang, Wei-Ping
Tang, Chun
description Solvent paramagnetic relaxation enhancement (sPRE) arises from random collisions between paramagnetic cosolvent and protein of interest. The sPRE can be readily measured, affording protein structure information. However, lack of an inert cosolvent probe may yield sPRE values that are not consistent with protein structure. Here we synthesized a new sPRE probe, triethylenetetraamine hexaacetate trimethylamide gadolinium, or Gd(III)–TTHA–TMA. With a total of 10 coordination sites, this paramagnetic cosovlent eliminates an inner-sphere water molecule that can otherwise transfer relaxation to protein through exchange. With the metal ion centered, the new probe is largely spherical with a radius of 4.0 Å, permitting accurate back calculation of sPRE. The effectiveness Gd(III)–TTHA–TMA as a sPRE probe was demonstrated on three well-studied protein systems.
doi_str_mv 10.1007/s10858-014-9817-3
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source Springer Nature
subjects Biochemistry
Biological and Medical Physics
Biophysics
Communication
Edetic Acid - analogs & derivatives
Edetic Acid - chemistry
Electron Spin Resonance Spectroscopy - methods
Gadolinium
Gadolinium - chemistry
Magnetic Resonance Spectroscopy
Nuclear Magnetic Resonance, Biomolecular - methods
Physics
Physics and Astronomy
Proteins - chemistry
Proteins - ultrastructure
Spectroscopy/Spectrometry
Water - chemistry
title A decadentate Gd(III)-coordinating paramagnetic cosolvent for protein relaxation enhancement measurement
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