Simultaneous Detection of Influenza Viruses A, B, and Swine Origin Influenza A Using Multiplex One-Step Real-Time RT-PCR Assay

Every year, seasonal epidemics of influenza viruses are causing considerable morbidity and mortality worldwide. Also infrequent novel and rearranged strains of influenza viruses have caused quick, acute universal pandemics resulting in millions of mortalities. The usage of efficient and accurate det...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 2014, Vol.172 (2), p.984-992
Main Authors: Monavari, S. H. R, Mollaie, H. R, Fazlalipour, M
Format: Article
Language:English
Subjects:
Animals
Biochemistry
Biological and medical sciences
Biotechnology
Chemistry
Chemistry and Materials Science
Diagnostic tests
disease control
Dogs
Fundamental and applied biological sciences. Psychology
Humans
Influenza
Influenza A Virus, H1N1 Subtype - genetics
Influenza A Virus, H1N1 Subtype - isolation & purification
Influenza B virus - genetics
Influenza B virus - isolation & purification
Influenza, Human - pathology
Influenza, Human - virology
Limit of Detection
Madin Darby Canine Kidney Cells
morbidity
mortality
Orthomyxoviridae
Orthomyxoviridae Infections - pathology
Orthomyxoviridae Infections - virology
pandemic
Pandemics
Polymerase chain reaction
quantitative polymerase chain reaction
Reagent Kits, Diagnostic
Real-Time Polymerase Chain Reaction - methods
reverse transcriptase polymerase chain reaction
Reverse Transcriptase Polymerase Chain Reaction - methods
Sensitivity and Specificity
swine
Swine - virology
Swine flu
swine influenza
viruses
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Summary:Every year, seasonal epidemics of influenza viruses are causing considerable morbidity and mortality worldwide. Also infrequent novel and rearranged strains of influenza viruses have caused quick, acute universal pandemics resulting in millions of mortalities. The usage of efficient and accurate detection is superior for infection control, effective treatment, and epidemiological supervision. Therefore, evaluation of useful real-time PCR molecular tests for the detection of pandemic viruses is important before the next wave of the pandemic. A novel quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR) assay with specific primers was used successfully for detection and monitoring of the influenza A, B, and swine influenza. The newly designed primers target highly conserved regions in influenza viruses. Our qRT-PCR assay is highly specific for detecting influenza A, B, and swine influenza viruses. The cutoff CT value was determined
ISSN:0273-2289
1559-0291
DOI:10.1007/s12010-013-0583-6