Photo-bio-synthesis of irregular shaped functionalized gold nanoparticles using edible mushroom Pleurotus florida and its anticancer evaluation

• Published in: Journal of photochemistry and photobiology. B, Biology Biology, 2013-08, Vol.125, p.63-69
• Main Authors: Bhat, Ravishankar, Sharanabasava, V.G., Deshpande, Raghunandan, Shetti, Ullas, Sanjeev, Ganesh, Venkataraman, A.
• Format: Article
• Language: English
• Subjects: adenocarcinoma; Agaricales; Animals; Anticancer evaluation; antineoplastic agents; Antineoplastic Agents - pharmacology; atomic force microscopy; Biofunctionalized gold nanoparticles; bone marrow; Cell Line, Tumor; Cell Survival - drug effects; Cercopithecus aethiops; cervix; Complex Mixtures - chemistry; Complex Mixtures - pharmacology; Complex Mixtures - radiation effects; energy-dispersive X-ray analysis; Fourier transform infrared spectroscopy; gold; Gold - pharmacology; Gold Compounds - chemistry; Gold Compounds - radiation effects; Green chemistry; Humans; ions; irradiation; kidneys; leukemia; lung neoplasms; mammary glands; Metal Nanoparticles; mushrooms; nanogold; nanoparticles; Photo-irradiation; photobiology; photochemistry; Pleurotus florida; Pleurotus ostreatus; scanning electron microscopy; Sunlight; transmission electron microscopy; ultraviolet-visible spectroscopy; Vero Cells; X-ray diffraction
• ISSN: 1011-1344; 1873-2682
• DOI: 10.1016/j.jphotobiol.2013.05.002

[Display omitted] •Photo-irradiation for gold nanoparticles production using mushroom P. folorida.•Biosynthetic method is advantageous being ecofriendly and nontoxic.•Nanoparticles are characterized using UV, XRD, AFM, TEM and FTIR.•Gold nanoparticles are potential for anticancer activity in vitro. A green chemistry approach to the synthesis of gold nanoparticles using edible mushroom Pleurotus florida (Oyster mushroom) by photo-irradiation method has been attempted. The mixture containing the aqueous gold ions and the mushroom extract was exposed to sunlight; this resulted in the formation of biofunctionalized gold nanoparticles. These nanoparticles were characterized using various techniques like UV–visible spectroscopy; X-ray diffraction studies, Energy dispersive X-ray analysis, Field emission scanning electron microscopy, Atomic force microscopy, Transmission electron microscopy and Fourier transform infrared spectrometry. The obtained biofunctionalized gold nanoparticles showed effective anti-cancer property against four different cancer cell lines A-549 (Human lung carcinoma), K-562 (Human chronic myelogenous leukemia bone marrow), HeLa (Human cervix) and MDA-MB (Human adenocarcinoma mammary gland) and no lethal effect is observed in Vero (African green monkey kidney normal cell) cell lines.