Rapid and sensitive detection of maize chlorotic mottle virus using surface plasmon resonance-based biosensor

We report a biosensor based on surface plasmon resonance (SPR) for the selective detection of maize chlorotic mottle virus (MCMV). 11-Mercaptoundecanoic acid was applied on a gold surface to form a self-assembled monolayer, and a layer of anti-MCMV antibody was crosslinked on the surface for specifi...

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Bibliographic Details
Published in:Analytical biochemistry 2013-09, Vol.440 (1), p.18-22
Main Authors: Zeng, Chang, Huang, Xin, Xu, Jiangmin, Li, Guifen, Ma, Jie, Ji, Hai-Feng, Zhu, Shuifang, Chen, Hongjun
Format: Article
Language:English
Subjects:
antibodies
Biosensing Techniques - methods
Biosensor
biosensors
crosslinking
detection limit
ELISA
Enzyme-Linked Immunosorbent Assay
Fatty Acids - analysis
gold
maize
Maize chlorotic mottle virus
Sensitivity and Specificity
Sulfhydryl Compounds - analysis
Surface plasmon resonance
Surface Plasmon Resonance - methods
Tombusviridae - isolation & purification
Virus detection
Zea mays - virology
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Summary:We report a biosensor based on surface plasmon resonance (SPR) for the selective detection of maize chlorotic mottle virus (MCMV). 11-Mercaptoundecanoic acid was applied on a gold surface to form a self-assembled monolayer, and a layer of anti-MCMV antibody was crosslinked on the surface for specific recognition of MCMV. The effects of coupling reaction time and antibody concentration on detection sensitivity were studied. The coverage mass change is a function of the concentration of MCMV with a dynamic range from 1 to 1000ppb. The detection limit is approximately 1ppb, which is approximately two orders of magnitude higher than that of the existing enzyme-linked immunosorbent assay (ELISA) method. The developed SPR sensor showed highly specific recognition for both purified MCMV and crude extracts from real-world samples.
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2013.04.026