Purification, characterization and immunostimulatory activity of polysaccharide from Cipangopaludina chinensis

• Published in: Carbohydrate polymers 2013-10, Vol.98 (1), p.217-223
• Main Authors: Xiong, Qingping, Jiao, Yunpeng, Zhao, Xirong, Chen, Xiaoming, Zhang, Qianghua, Jiang, Changxing
• Format: Article
• Language: English
• Subjects: Adjuvants, Immunologic - chemistry; Adjuvants, Immunologic - isolation & purification; Adjuvants, Immunologic - pharmacology; Animals; Applied sciences; Biological and medical sciences; blood serum; chromatography; Cipangopaludina chinensis; Cyclophosphamide - pharmacology; Exact sciences and technology; Gastropoda - chemistry; glucose; Hemolysin Proteins - blood; hemolysins; Immunomodulators; Immunostimulatory activity; macrophages; Macrophages - drug effects; Macrophages - immunology; Male; Medical sciences; Mice; Molecular Weight; Natural polymers; Pharmacology. Drug treatments; Physicochemistry of polymers; polysaccharides; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; Preliminary characterization; Purification; rhamnose; spleen; Spleen - drug effects; Spleen - immunology; Starch and polysaccharides; sulfuric acid; thymus gland; Thymus Gland - drug effects; Thymus Gland - immunology; uronic acids
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2013.05.075

•Polysaccharide from C. chinensis (CCPS) was purified.•Preliminary characterization of CCPS was investigated.•CCPS showed a significant immunostimulatory effect in vivo. In this study, we investigated the purification, preliminary characterization and immunostimulatory activity in vivo of polysaccharide from Cipangopaludina chinensis (CCPS). Firstly, crude CCPS was prepared by hot water extraction. And the crude CCPS was sequentially purified by chromatography of DEAE-52 and Sephadex G-100, resulting in two purified fractions of CCPS-1 and CCPS-2. We found the two fractions were homogeneous heteropolysaccharides mainly composed of rhamnose and glucose with the average molecular weight of 226 and 235kDa, respectively. CCPS-2 was quite different from CCPS-1. It had much higher content of uronic acid and sulfuric radical. For immunostimulatory activity in vivo, crude CCPS could significantly increase the thymus and spleen indices, enhance the macrophage function, and increase the level of serum hemolysin in cyclophosphamide-treated mice, suggesting CCPS had a potent immunostimulatory activity and could be explored as a potential natural immunomodulatory agent