Immunoenhancement effect of rehmannia glutinosa polysaccharide on lymphocyte proliferation and dendritic cell

• Published in: Carbohydrate polymers 2013-07, Vol.96 (2), p.516-521
• Main Authors: Huang, Yee, Jiang, Chunmao, Hu, Yuanliang, Zhao, Xiaojuan, Shi, Cheng, Yu, Yun, Liu, Cui, Tao, Yang, Pan, Huarong, Feng, Yibo, Liu, Jiaguo, Wu, Yi, Wang, Deyun
• Format: Article
• Language: English
• Subjects: Animals; Antigen Presentation - drug effects; antigens; Applied sciences; Biological and medical sciences; bone marrow; Cell Proliferation - drug effects; coculture; Dendritic cell; dendritic cells; Dendritic Cells - drug effects; Dendritic Cells - immunology; enzyme-linked immunosorbent assay; Exact sciences and technology; IFN-γ; IL-2; Immunoenhancement; Immunologic Factors - pharmacology; Immunomodulators; interferon-gamma; Interferon-gamma - genetics; Interferon-gamma - metabolism; interleukin-2; Interleukin-2 - genetics; Interleukin-2 - metabolism; Lymphocyte proliferation; Medical sciences; Mice; Natural polymers; Pharmacology. Drug treatments; Physicochemistry of polymers; polysaccharides; Polysaccharides - pharmacology; Rehmannia - chemistry; Rehmannia glutinosa; Rehmannia glutinosa polysaccharide; Starch and polysaccharides; T-lymphocytes; T-Lymphocytes - drug effects; T-Lymphocytes - metabolism
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2013.04.018

•RGP significantly stimulated proliferation of murine splenic lymphocyte.•RGP significantly upregulated IL-2 and IFN-γ production of T lymphocyte.•RGP could improve DCs stimulating on proliferation of T cells.•RGP could enhance the ability of antigen presenting of DCs. The aim of this study is to investigate immunomodulatory effect of rehmannia glutinosa polysaccharide (RGP) on murine splenic lymphocyte and bone marrow derived dendritic cells (DCs). Splenic lymphocytes obtained from mice were co-cultured with RGP for 48h and then harvested for analyzing with MTT method. The cytokine production of T lymphocytes was measured by ELISA. Effects of RGP treatment on DCs were investigated and assessed by MTT method. The results showed RGP significantly stimulated lymphocyte proliferation and the growth rate of T cell was more significant. The IL-2 and IFN-γ production of T lymphocyte were significantly upregulated after being stimulated with RGP. DCs stimulating on proliferation of T cells and the ability of antigen presenting of DCs have been enhanced under the stimulation of RGP. In conclusion, these findings provided valuable information that RGP possessed strong immunoenhancement activity, which provided the theoretical basis for the further experiment.