Improvement of brain uptake for in vivo PET imaging of astrocytic oxidative metabolism using benzyl [1-11C]acetate

Brain uptake of acetate is insufficient for obtaining a quantitative image of astrocytic oxidative metabolism. To improve the brain uptake of [1-11C]acetate, we synthesized benzyl [1-11C]acetate ([1-11C]BA) and conducted a positron emission tomography (PET) study assessing astrocytic oxidative metab...

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Bibliographic Details
Published in:Applied radiation and isotopes 2013-08, Vol.78, p.102-107
Main Authors: Okada, Maki, Nakao, Ryuji, Momosaki, Sotaro, Yanamoto, Kazuhiko, Kikuchi, Tatsuya, Okamura, Toshimitsu, Wakizaka, Hidekatsu, Hosoi, Rie, Zhang, Ming-Rong, Inoue, Osamu
Format: Article
Language:English
Subjects:
[1-11C]acetate
Acetates - pharmacokinetics
Animals
Astrocyte
Astrocytes - diagnostic imaging
Astrocytes - metabolism
Benzyl[11C]acetate
Brain - diagnostic imaging
Brain - metabolism
Carbon - pharmacokinetics
Energy metabolism
Male
Metabolic Clearance Rate
Positron emission tomography
Positron-Emission Tomography - methods
Radiopharmaceuticals - pharmacokinetics
Rats
Rats, Sprague-Dawley
Reproducibility of Results
Sensitivity and Specificity
Tissue Distribution
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Summary:Brain uptake of acetate is insufficient for obtaining a quantitative image of astrocytic oxidative metabolism. To improve the brain uptake of [1-11C]acetate, we synthesized benzyl [1-11C]acetate ([1-11C]BA) and conducted a positron emission tomography (PET) study assessing astrocytic oxidative metabolism. The brain uptake of [1-11C]BA was markedly higher compared with [1-11C]acetate, and disappeared with a half-life of 20min in all regions studied. The brain uptake of [1-11C]BA was significantly decreased by fluorocitrate. The results indicate that [1-11C]BA could be a useful PET probe for assessing astrocytic oxidative metabolism. •The measurement of astrocytic oxidative metabolism by PET.•The brain uptake of benzyl [1-11C]acetate was higher than that of [1-11C]acetate.•The uptake of benzyl [1-11C]acetate was inhibited by fluorocitrate.•Benzyl [1-11C]acetate is a useful PET probe for astrocytic oxidative metabolism.
ISSN:0969-8043
1872-9800
DOI:10.1016/j.apradiso.2013.04.025