Overexpression of constitutively active OsCPK10 increases Arabidopsis resistance against Pseudomonas syringae pv. tomato and rice resistance against Magnaporthe grisea

• Published in: Plant physiology and biochemistry 2013-12, Vol.73, p.202-210
• Main Authors: Fu, Liwen, Yu, Xiangchun, An, Chengcai
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Arabidopsis; Arabidopsis - enzymology; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis - microbiology; Bacterial plant pathogens; Biological and medical sciences; calcium; Calcium-Binding Proteins - genetics; Calcium-Binding Proteins - metabolism; casein; CDPK; Cyclopentanes - metabolism; disease resistance; Disease Resistance - genetics; elicitors; Fundamental and applied biological sciences. Psychology; Fungal plant pathogens; fungi; Gene Expression Regulation, Plant; gene overexpression; genes; Genes, Plant; genetically modified organisms; immune response; Lycopersicon esculentum; Magnaporthe; Magnaporthe grisea; Molecular Sequence Data; Oryza - enzymology; Oryza - genetics; Oryza - metabolism; Oryza - microbiology; Oryza sativa; Oxylipins - metabolism; pathogens; Phytopathology. Animal pests. Plant and forest protection; Plant Diseases - microbiology; Plant immunity; Plant physiology and development; Plant Proteins - genetics; Plant Proteins - metabolism; plant response; Plants, Genetically Modified; protein kinases; Protein-Serine-Threonine Kinases - genetics; Protein-Serine-Threonine Kinases - metabolism; Pseudomonas syringae; Pseudomonas syringae pv. tomato; rice; Salicylic Acid - metabolism
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2013.10.004

Calcium-dependent protein kinases (CDPKs) are crucial calcium sensors involved in plant responses to pathogen infection. Here, we report isolation and functional characterization of the pathogen-responsive rice OsCPK10 gene. The expression of OsCPK10 was strongly induced following treatment with a Magnaporthe grisea elicitor. Kinase activity assay showed that the functional OsCPK10 protein not only autophosphorylated, but also phosphorylated Casein in a calcium-dependent manner. Overexpression of constitutively active OsCPK10 in Arabidopsis enhanced the resistance to infection with Pseudomonas syringae pv. tomato, associated with elevated expression of both SA- and JA-related defense genes. Similarly, transgenic rice plants containing constitutively active OsCPK10 exhibited enhanced resistance to blast fungus M. grisea. The enhanced resistance in the transgenic lines was associated with activated expression of SA- and JA-related defense genes. Collectively, our results indicate that rice OsCPK10 is a crucial regulator in plant immune responses, and that it may regulate disease resistance by activating both SA- and JA-dependent defense responses. •We isolated a pathogen-responsive CDPK in rice.•OsCPK10 positively regulate disease resistance in plants.•OsCPK10 may activate both SA- and JA-dependent defense responses.