In Vivo and In Vitro Evidence for Placental DNA Damage in Preeclampsia: e86791

Preeclampsia (PE) is an idiopathic multisystem disease affecting 5-7% of pregnant women. Placental oxidative stress is a characteristic feature of PE and occurs when the production of reactive oxygen species (ROS) within the placenta overwhelms the intrinsic anti-oxidant defenses. We hypothesize tha...

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Bibliographic Details
Published in:PloS one 2014-01, Vol.9 (1)
Main Authors: Tadesse, Serkalem, Kidane, Dawit, Guller, Seth, Luo, Tianmeng, Norwitz, Nicholas G, Arcuri, Felice, Toti, Paolo, Norwitz, Errol R
Format: Article
Language:English
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Summary:Preeclampsia (PE) is an idiopathic multisystem disease affecting 5-7% of pregnant women. Placental oxidative stress is a characteristic feature of PE and occurs when the production of reactive oxygen species (ROS) within the placenta overwhelms the intrinsic anti-oxidant defenses. We hypothesize that excessive oxidative DNA damage at the fetal-maternal interface coupled with a defective DNA damage/repair response is causally related to PE. Here we demonstrate that gamma H2AX (a sensitive marker of DNA damage) is expressed in the maternal decidua but not trophoblast of normal placentas, and that expression is significantly higher in PE placental tissues in vivo. Using primary in vitro cultures of maternal decidual stromal cells (DSCs) and fetal cytotrophoblast cells (CTs), we show an increase in gamma H2AX foci in DSCs cultured with vs without H2O2 (70.6% vs 11.6%; P
ISSN:1932-6203
DOI:10.1371/journal.pone.0086791