Evaluation of in vitro antioxidant potency of exopolysaccharide from endophytic Fusarium solani SD5

• Published in: International journal of biological macromolecules 2013-02, Vol.53, p.62-66
• Main Authors: Mahapatra, Subhadip, Banerjee, Debdulal
• Format: Article
• Language: English
• Subjects: 2,2-diphenyl-1-picrylhydrazyl; Animals; Antioxidant activity; Ascorbic Acid - chemistry; Biphenyl Compounds - chemistry; Cell Survival - drug effects; Cells, Cultured; Cytotoxicity; endophytes; Endophytes - chemistry; Endophytic Fusarium solani SD5; Exopolysaccharide; exopolysaccharides; free radical scavengers; Free Radical Scavengers - chemistry; Free Radical Scavengers - isolation & purification; Free Radical Scavengers - toxicity; free radicals; Free Radicals - chemistry; Fungal Polysaccharides - chemistry; Fungal Polysaccharides - isolation & purification; Fungal Polysaccharides - toxicity; fungi; Fusarium - chemistry; Fusarium solani; glutathione; Glutathione - metabolism; glutathione peroxidase; Glutathione Peroxidase - metabolism; in vitro studies; inhibitory concentration 50; Lipid Peroxidation; macrophages; Macrophages, Peritoneal - drug effects; Macrophages, Peritoneal - physiology; Male; malondialdehyde; Malondialdehyde - metabolism; Mice; oxidants; Peritoneal macrophage; Picrates - chemistry; protective effect; superoxide dismutase; Superoxide Dismutase - metabolism; Tetrazolium Salts - chemistry; Thiazoles - chemistry
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2012.11.003

A potent endophytic fungus, Fusarium solani SD5 was used for exopolysaccharide (EPS) production. The isolated EPS were purified and major EPS fraction (PS-I); rhamno galactan was used to evaluate anti oxidant activities in vitro. EPS (PS-I) showed significant free radical scavenging effect on DPPH (1,1-diphenyl-2-picrylhydrazyl) and scavenging potency is indicated by IC50 value 578.541±33.256μg/ml. EPS (PS-I) significantly induced antioxidant parameters of peritoneal macrophage cells at a concentration dependent manner and at 500μg/ml it showed maximum protective effect against free radicals [malondialdehyde (MDA) 0.178±0.015; super oxide dismutase (SOD) 41.287±1.051; glutathione peroxidase (GPx) 30.182±1.237; reduced glutathione (GSH) 56.892±1.272; oxidized glutathione (GSSG) 8.458±0.768]. MTT [3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide] cytotoxicity assay indicated that EPS (PS-I) had no significant cytotoxic effect (concentration up to 500μg/ml) on macrophage cells. Present findings suggested that the EPS (PS-I) may become a potential nontoxic exogenous antioxidant.