Biodesulfurization of Model Compounds and De-asphalted Bunker Oil by Mixed Culture

In this study, complicated model sulfur compounds in bunker oil and de-asphalted bunker oil were biodesulfurized in a batch process by microbial consortium enriched from oil sludge. Dibenzothiophene (DBT) and benzo[b]naphtho[1,2-d]thiophene (BNT1) were selected as model sulfur compounds. The results...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 2014, Vol.172 (1), p.62-72
Main Authors: Jiang, Xia, Yang, Senlin, Li, Wangling
Format: Article
Language:English
Subjects:
Bacteria - cytology
Bacteria - drug effects
Bacteria - growth & development
Bacteria - metabolism
Biocatalysis
Biochemistry
Biological and medical sciences
Biotechnology
Cell Proliferation - drug effects
Chemistry
Chemistry and Materials Science
Coculture Techniques
Complex systems
Desulfurization
Fundamental and applied biological sciences. Psychology
Hydrocarbons - chemistry
Metabolites
mixed culture
mixing
Oil
Petroleum - microbiology
Sewage - microbiology
Sludge
Sulfur
Sulfur content
thiophene
Thiophenes - metabolism
Thiophenes - pharmacology
viscosity
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Summary:In this study, complicated model sulfur compounds in bunker oil and de-asphalted bunker oil were biodesulfurized in a batch process by microbial consortium enriched from oil sludge. Dibenzothiophene (DBT) and benzo[b]naphtho[1,2-d]thiophene (BNT1) were selected as model sulfur compounds. The results show that the mixed culture was able to grow by utilizing DBT and BNT1 as the sole sulfur source, while the cell density was higher using DBT than BNT1 as the sulfur source. GC-MS analysis of their desulfurized metabolites indicates that both DBT and BNT1 could be desulfurized through the sulfur-specific degradation pathway with the selective cleavage of carbon–sulfur bonds. When DBT and BNT1 coexisted, the biodesulfurization efficiency of BNT1 decreased significantly as the DBT concentrations increased (>0.1 mmol/L). BNT1 desulfurization efficiency also decreased along with the increase of 2-hydroxybiphenyl as the end product of DBT desulfurization. For real bunker oil, only 2.8 % of sulfur was removed without de-asphalting after 7 days of biotreatment. After de-asphalting, the biodesulfurization efficiency was significantly improved (26.2–36.5 %), which is mainly attributed to fully mixing of the oil and water due to the decreased viscosity of bunker oil.
ISSN:0273-2289
1559-0291
DOI:10.1007/s12010-013-0494-6