Early hepatic and peritoneal changes and immune response in goats vaccinated with a recombinant glutathione transferase sigma class and challenged with Fasciola hepatica

• Published in: Research in veterinary science 2013-06, Vol.94 (3), p.602-609
• Main Authors: Zafra, R., Pérez-Écija, R.A., Buffoni, L., Pacheco, I.L., Martínez-Moreno, A., LaCourse, E.J., Perally, S., Brophy, P.M., Pérez, J.
• Format: Article
• Language: English
• Subjects: Adjuvants, Immunologic - therapeutic use; Animals; Cattle; Disease; eosinophils; Fasciola hepatica; Fasciola hepatica - immunology; Fascioliasis - immunology; Fascioliasis - parasitology; Fascioliasis - pathology; Fascioliasis - prevention & control; Fascioliasis - veterinary; flukes; Glutathione transferase; Glutathione Transferase - genetics; Glutathione Transferase - immunology; Goat Diseases - immunology; Goat Diseases - parasitology; Goat Diseases - pathology; Goat Diseases - prevention & control; Goats; Goats - immunology; Goats - parasitology; immune response; Immunity, Cellular - immunology; Immunity, Humoral - immunology; inducible nitric oxide synthase; Infections; interferon-gamma; interleukin-4; larvae; Liver; Liver - parasitology; Liver - pathology; Male; necrosis; Nitric oxide; Parasites; Peritoneum - parasitology; Peritoneum - pathology; Proteins; Quillaja Saponins; Saponins - therapeutic use; T-lymphocytes; Trematoda; vaccination; Vaccine; Vaccines - immunology; Vaccines - therapeutic use; Vaccines, Synthetic - immunology; Vaccines, Synthetic - therapeutic use; Veterinary medicine
• ISSN: 0034-5288; 1532-2661
• DOI: 10.1016/j.rvsc.2012.10.026

Changes and local immune response were evaluated in the peritoneal cell populations, duodenal lamina propria and liver from goats immunized with recombinant glutathione transferase sigma class (rFhGST-S1) during early stages of infection with Fasciola hepatica. Group 1 (n=7) was unimmunized and uninfected; group 2 (n=10) was immunized with adjuvant Quil A and infected; group 3 (n=10) was immunised with rFhGST-S1 and infected. Three goats from each group were killed at 7–9days post-infection (dpi) to evaluate early changes and immune response. The remaining goats were killed at 15weeks post-infection (wpi). rFhGST-S1 vaccination induced variable response: three goats showed low fluke burden at 15 wpi and two goats showed low hepatic damage at early infection stages. This response was associated to a severe infiltrate of eosinophils in peritoneal fluid and hepatic necrotic foci, high iNOS expression in peritoneal cells and abundant infiltrate of eosinophils surrounding hepatic migrating flukes. T lymphocyte subsets were found in the vicinity of necrotic areas but they were absent in the vicinity of migrating larvae. No significant variation for T cell subsets, except for CD4 and γδ T lymphocytes, that were higher in the Quil A group compared to the rFhGST-S1 group. Expression of IL4 and IFN-γ in the hepatic inflammatory infiltrates was very occasional.