ATP formation coupled to caffeate reduction by H sub(2) in Acetobacterium woodii) NZva16

The authors have addressed the question, whether the reduction of caffeate in Acetobacterium woodii strain NZva16 is coupled to ATP synthesis by electron transport phosphorylation. The following results were obtained: 1. Cultures of A. woodii with H sub(2) and CO sub(2), grew to greater cell densiti...

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Bibliographic Details
Published in:Archives of microbiology 1988-01, Vol.150 (5), p.447-451
Main Authors: Hansen, B, Bokranz, M, Schoenheit, P, Kroeger, A
Format: Article
Language:English
Subjects:
Acetobacterium woodii
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Summary:The authors have addressed the question, whether the reduction of caffeate in Acetobacterium woodii strain NZva16 is coupled to ATP synthesis by electron transport phosphorylation. The following results were obtained: 1. Cultures of A. woodii with H sub(2) and CO sub(2), grew to greater cell densities, when caffeate was also present. 2. Non-growing bacterial suspensions catalyzed the reduction of caffeate by H sub(2). 3. The ATP content of bacteria incubated with H sub(2) increased from < 1 to about 7 mu mol per g cellular protein on the addition of caffeate. 4. Caffeate in the presence of H sub(2) caused the uptake of tetraphenylphosphonium cation by the bacteria. It is concluded that caffeate reduction by H sub(2) is coupled to ATP formation by electron transport phosphorylation. However, the failure of protonophores to prevent phosphorylation and TPP uptake cannot be explained.
ISSN:0302-8933