Fabrication of a Colorimetric Carcino-Embryonic Antigen Sensor Using High-Activity DNAzyme as a Catalytic Label

Detection of biomarkers in a biologically complex mixture remains a major challenge. Herein, an ultrasensitive colorimetric sandwich sensor for carcino-embryonic antigen (CEA) detection is introduced. The DNAzyme was tethered to biotinylated monoclonal antibodies (McAbs) which serve as the sensing e...

Full description

Saved in:
Bibliographic Details
Published in:Analytical letters 2013-09, Vol.46 (13), p.2040-2047
Main Authors: Guo, Xuedan, Huang, Qing, Lin, Yina
Format: Article
Language:English
Subjects:
Antigens
Biomarkers
Cancer biomarker
Carcino-embryonic antigen (CEA)
Colorimetric sensor
Colorimetry
G-quadruplexes
Immunoassay
Mathematical analysis
Recognition
Sensors
Strategy
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Detection of biomarkers in a biologically complex mixture remains a major challenge. Herein, an ultrasensitive colorimetric sandwich sensor for carcino-embryonic antigen (CEA) detection is introduced. The DNAzyme was tethered to biotinylated monoclonal antibodies (McAbs) which serve as the sensing element to recognize the target protein and was then introduced on to the CEA-McAbs assembled micro plate. The CEA was captured in a sandwich assay by the McAbs. The peroxidase-like DNAzyme catalyzed the oxidation of 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), which generated a blue-green colorimetric signal. This method detected CEA in a serum-containing medium at a concentration as low as 10 nM. This strategy is a promising tool for bioanalytical and clinical applications.
ISSN:0003-2719
1532-236X
DOI:10.1080/00032719.2013.784917